OXPHOSight™ ATP Assay Kit

CAT#: ITS-1125-YL24
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Measures ATP levels using a bioluminescent method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ ATP Assay Kit provides a bioluminescent method for the rapid quantification of cellular energy levels in diverse biological matrices. Utilizing the firefly luciferase system, the assay produces a light signal proportional to the ATP concentration with exceptional sensitivity. This high-throughput tool is essential for researchers evaluating metabolic activity, cell viability, and mitochondrial function, ensuring reliable results for broad physiological and biochemical investigations.
Features
Employs a bioluminescent luciferase-based reaction for ultra-sensitive ATP quantification, serving as an essential tool for monitoring cellular energy status and metabolic viablity in research.
Process Relevance
Measuring ATP concentration to monitor cellular bioenergetics and ensure metabolic consistency in studies.
Application Stage
Cell viablity screening and energy metabolism research.
Applications
Measure cellular energy levels and metabolic activity in diverse biological samples using highly sensitive luminescence-based detection methodologies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Luminescence
Sample Type
Cell lysates, tissue homogenates.
Research Areas
Bioenergetics; Cell viablity; Metabolic Research
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
10 min
LOD
LOD: 0.02 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Adenosine triphosphate is the universal energy currency of the cell and a key signaling molecule. Its levels are a primary indicator of metabolic activity and cell viablity, making its measurement central to many areas of biological research.
Pathways
Sialic acid is an essential component of the glycan structures on cell surfaces, influencing downstream events like cell adhesion and pathogen entry. It is synthesized via the hexosamine pathway and added to proteins in the Golgi apparatus.
For Research Use Only | Not For Clinical Use
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