OXPHOSight™ NAD/NADH Assay Kit

CAT#: ITS-1125-HM115
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Cycling assay for total NAD and NADH determination.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ NAD/NADH Assay Kit uses a cycling enzyme reaction to amplify signals, allowing for the quantification of cofactor ratios in small cell pellets. This assay validates the cellular redox state, which is a central regulator of metabolism and sirtuin activity. It supports research into aging, cancer, and metabolic diseases by measuring the availability of oxidizing equivalents.
Features
Cycling enzyme reaction; Amplified signal allows quantification of cofactor ratios in small cell pellets.
Process Relevance
Cellular Redox Status
Application Stage
Discovery / Mechanism of Action
Applications
Cellular redox state and metabolic cofactor analysis.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Cell or tissue extracts
Research Areas
Aging Research; Metabolic Disease
Size
100 tests
Data Output Type
Quantitative (Ratio / Concentration)
Assay Time
Results in 15 min
LOD
0.05 µM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
The NAD+/NADH ratio determines the cellular redox state and drives oxidative phosphorylation. Disruptions in this ratio are hallmarks of metabolic disease aging and cancer.
For Research Use Only | Not For Clinical Use
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