Short Description
Cycling assay for total NAD and NADH determination.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ NAD/NADH Assay Kit uses a cycling enzyme reaction to amplify signals, allowing for the quantification of cofactor ratios in small cell pellets. This assay validates the cellular redox state, which is a central regulator of metabolism and sirtuin activity. It supports research into aging, cancer, and metabolic diseases by measuring the availability of oxidizing equivalents.
Features
Cycling enzyme reaction; Amplified signal allows quantification of cofactor ratios in small cell pellets.
Process Relevance
Cellular Redox Status
Application Stage
Discovery / Mechanism of Action
Applications
Cellular redox state and metabolic cofactor analysis.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Cell or tissue extracts
Research Areas
Aging Research; Metabolic Disease
Datasheet