Phosphatase Panel Screening Service
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The Critical Role of Phosphatases in Biological Systems
Phosphatases, enzymes responsible for removing phosphate groups from various substrates, play indispensable roles in regulating nearly every aspect of cellular life. Their counteractive roles to kinases ensure the precise control of signal transduction pathways, influencing cell proliferation, differentiation, metabolism, gene expression, and apoptosis. Dysregulation of phosphatase activity is intricately linked to the etiology and progression of numerous human diseases, including cancers, metabolic disorders like diabetes, neurological conditions, and inflammatory diseases. Consequently, phosphatases have emerged as significant therapeutic targets, prompting intensive efforts in drug discovery and development.
Fig.1 Roles of Alkaline Phosphatases in bone and cardiovascular disease.1
Our Phosphatase Panel Screening Service
At Creative Biolabs, our Phosphatase Panel Screening Service is meticulously designed to provide comprehensive and high-fidelity assessment of phosphatase enzyme activity. Leveraging our two decades of expertise in enzyme kinetics and high-throughput screening, we offer a robust platform for researchers and pharmaceutical companies seeking to elucidate phosphatase function or identify modulators of their activity. This service is a cornerstone of our commitment to accelerating drug discovery and advancing fundamental biological understanding.
Our service extends beyond mere activity measurement, delving into detailed kinetic characterization and selectivity profiling. By precisely quantifying the dephosphorylation events, we empower our clients to gain deeper insights into the intricate mechanisms governing phosphatase function and their interactions with potential inhibitors or activators. The versatility of our platform allows for the analysis of various sample types, ranging from purified recombinant proteins to complex cell or tissue extracts, ensuring broad applicability across diverse research paradigms.
Service Content and Workflow
Creative Biolabs' Phosphatase Panel Screening Service is structured to deliver accurate, reproducible, and timely results through a well-defined workflow.
Comprehensive Panel Selection:
We offer access to a diverse panel of phosphatase enzymes, encompassing major classes such as:
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Alkaline Phosphatases (ALPs): Often indicative of liver or bone pathologies, their activity is crucial for various physiological processes.
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Acid Phosphatases (ACPs): Historically significant in prostate cancer diagnostics, although their role extends to lysosomal function and bone metabolism.
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Protein Tyrosine Phosphatases (PTPs): These enzymes are critical regulators of tyrosine phosphorylation, impacting numerous signaling cascades related to cell growth, differentiation, and immune responses. They represent highly attractive drug targets.
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Serine/Threonine Phosphatases: Essential for regulating the phosphorylation status of serine and threonine residues, these phosphatases are pivotal in controlling cell cycle progression, gene expression, and metabolic pathways.
Clients can select from a pre-defined broad panel or customize a focused panel based on their specific research objectives and target phosphatases of interest.
Flexible Sample Handling:
Creative Biolabs accommodates a wide range of sample types, including:
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Purified recombinant phosphatases
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Cellular lysates
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Tissue extracts
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Crude biological samples
Our robust assay conditions are optimized to minimize matrix effects and ensure consistent enzyme activity measurements across diverse sample matrices.
Data Acquisition and Analysis:
High-throughput screening platforms are employed to process large compound libraries efficiently. The fluorescent signal, directly proportional to phosphatase activity (or inhibition), is continuously monitored, providing comprehensive kinetic profiles. Data are rigorously analyzed to determine key parameters such as:
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% Inhibition/Activation: For initial screening of compound libraries.
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IC50/EC50 Values: Precise quantification of compound potency.
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Kinetic Parameters (e.g., Ki, Km): For detailed mechanistic studies of lead compounds.
Comprehensive Reporting:
Creative Biolabs provides detailed reports including raw data, processed results, kinetic curves, and statistical analyses, all delivered in a clear and accessible format. Our scientific team is available for in-depth discussions to interpret results and guide subsequent research directions.
Our Advantages
Creative Biolabs stands at the forefront of phosphatase research and drug discovery, offering distinct advantages that set our service apart:
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Decades of Specialized Expertise
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Advanced Assay Technology
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High-Throughput Capabilities
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Quality and Reproducibility
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Customization and Flexibility
FAQ
Q1: What types of compounds can be screened using Creative Biolabs' service?
A1: Our service is designed to screen a wide range of compounds, including small molecules, peptides, and natural products, for their inhibitory or activating effects on phosphatase activity.
Q2: Can I provide my own phosphatase enzyme or substrate?
A2: Yes, Creative Biolabs offers flexibility in incorporating client-supplied enzymes or substrates into our screening workflows. Our team will work with you to ensure compatibility and optimal assay conditions.
Q3: What is the typical turnaround time for a Phosphatase Panel Screen?
A3: The turnaround time can vary depending on the scope and scale of the project. However, our optimized workflows and high-throughput capabilities ensure rapid delivery of results, with initial screening data often available within a few weeks.
Q4: How does Creative Biolabs ensure the selectivity of compounds identified?
A4: Our comprehensive phosphatase panels and detailed kinetic profiling allow for thorough selectivity assessment. We can further design follow-up assays to evaluate compound specificity against closely related phosphatases or other enzyme classes, providing a clear selectivity profile.
Q5: Are your assays compatible with complex biological samples?
A5: Yes, our assay systems are robustly optimized for use with various biological matrices, including cell lysates and tissue extracts, while minimizing interference. Our team can assist with sample preparation protocols to ensure optimal results.
Contact Us
For further inquiries or to discuss your specific Phosphatase Panel Screening needs, please do not hesitate to contact our dedicated team of specialists. We are committed to providing exceptional scientific support and tailored solutions to accelerate your research and drug discovery initiatives. At Creative Biolabs, we are your trusted partner in unlocking the therapeutic potential of phosphatase modulation.
Reference
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Haarhaus, Mathias et al. "Alkaline Phosphatase: An Old Friend as Treatment Target for Cardiovascular and Mineral Bone Disorders in Chronic Kidney Disease." Nutrients vol. 14,10 2124. 19 May. 2022, doi:10.3390/nu14102124. Distributed under Open Access License CC BY 4.0, without modification.
