The kit is designed for in vitro quantitative measurement of Pig IL-10 (IL10) in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA).
Microplate reader. Pipettes and pipette tips. EP tube Deionized or distilled water.
Sensitivity
7 pg/mL
Sample Volume
20 μL
Assay Time
3 h
Plate
Pre-coated
Storage
4 °C
Restrictions
For Research Use only
Background
Porcine IL-10 is a secreted, possibly glycosylated, polypeptide with an 18 kDa molecular weight. Based on human studies, porcine IL-10 is likely to circulate as a nondisulfide-linked homodimer. Porcine IL-10 is synthesized as a 175 amino acid (aa) precursor with an 18 aa signal sequence and a 157 aa mature form. The mature segment has one potential N-linked glycosylation site plus four cysteines which form two intrachain disulfide bridges.
UniProt
A0A5S6GG93
Pathways
Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints