SigPath™ Human/Mouse 4EBP1(S65) Phosphorylation ELISA Kit (CAT#: ITS-1125-WB6)

Quantifies 4E-BP1 (Ser65) phosphorylation levels in cell lysates using an indirect ELISA method.

SigPath

Research-Grade Signal Pathway Assay Development

The SigPath™ Human/Mouse 4EBP1(S65) Phosphorylation ELISA Kit provides a rapid and sensitive solution for quantifying p4EBP1(S65) levels in cultured cells. This cell-based system eliminates lysate preparation, featuring in-well total protein normalization to ensure high precision. It is an essential tool for high-throughput screening of inhibitors and activators within the mTOR signaling pathway.

High-Throughput Cell-Based Format: Directly culture and assay cells in 96-well plates, eliminating the need for laborious cell lysate preparation.
Internal Protein Normalization: Total cellular protein is measured in the same well to correct for variations in cell loading and density.
Rapid Diagnostic Workflow: Total assay time is reduced to 6.5 hours, requiring only 2.5 hours of hands-on technical time for completion.
Dual-Species Reactivity: Validated for both human and mouse samples, ensuring high specificity for the S65 phosphorylation site of the 4EBP1 protein.

Monitoring 4EBP1(S65) phosphorylation levels to validate drug-induced mTOR pathway modulation and assess translation initiation efficiency in cellular models.

Preclinical drug discovery, high-throughput inhibitor screening, and signal transduction research.

Quantifying p4EBP1(S65) in adherent or suspension cells to evaluate the potency of mTOR/PI3K inhibitors, activators, or siRNA-mediated gene silencing effects during lead optimization.

Internal performance validation using reference standards under defined assay conditions.

Phosphorylated serine 65 (S65) of the eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1).

Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 452 nm)

Cells

Oncology therapeutics, metabolic diseases, pharmacokinetics, protein synthesis regulation, etc.