SigPath™ Human/Mouse AKT1(S473) Phosphorylation ELISA Kit

CAT#: ITS-1125-WB7
Product Type: Assay Kit
Species: Human, mouse, rat
Target: Specifically detects phosphorylated human or mouse AKT1 at the serine 473 residue.
Brand: SigPath
Development Stage: Research-Grade Signal Pathway Assay Development
Short Description
Quantifies Akt (S473) phosphorylation in whole cells using an indirect ELISA method.
Brand
SigPath
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ Human/Mouse AKT1(S473) Phosphorylation ELISA Kit offers a high-throughput solution for monitoring the PI3K/Akt signaling pathway by quantifying pAKT1(S473) levels directly in cultured cells. By integrating total protein normalization within the same well, this cell-based system eliminates lysate preparation and minimizes experimental variability, facilitating precise screening of kinase inhibitors and activators in drug discovery.
Features
High-Precision Detection: This assay ensures exceptional sensitivity and specificity for phosphorylated Serine 473, minimizing cross-reactivity with non-phosphorylated Akt isoforms.
Streamlined Workflow: The high-throughput microplate format includes pre-coated surfaces and optimized reagents to reduce hands-on time and experimental variability.
Robust Reproducibility: Rigorous quality control guarantees consistent performance across different batches, providing reliable quantitative data for longitudinal research studies.
Species Versatility: Validated for both human and murine cell lysates, allowing for seamless transition between clinical models and preclinical animal studies.
Process Relevance
Quantifying Akt activation to evaluate kinase inhibitor efficacy and characterize therapeutic modulation of survival-related signaling cascades.
Application Stage
Drug discovery, preclinical candidate validation, and molecular mechanism-of-action profiling.
Applications
Ideal for cell-based screening, kinase assays, and monitoring AKT1 activation in response to growth factors or pharmacological inhibitors using various mammalian cell lysates.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Specifically detects phosphorylated human or mouse AKT1 at the serine 473 residue.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 453 nm)
Sample Type
Cells
Research Areas
Oncology therapeutics, metabolic disorders, immunology, neurobiology, regenerative medicine, etc.
Size
100 tests
Data Output Type
Quantitative measurement of relative phosphorylation levels via endpoint colorimetric absorbance.
Assay Time
6.5 h
Species
Human, mouse, rat
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Akt serves as a central node in eukaryotic signaling, regulating growth and metabolism. Phosphorylation at Ser473 by mTORC2 is a prerequisite for its full catalytic activation and downstream function.
Pathways
This protein is the cornerstone of the PI3K/Akt/mTOR pathway. Activated by upstream phosphoinositide signaling, it modulates downstream effectors like GSK3β and FOXO to govern cell survival and proliferation.
For Research Use Only | Not For Clinical Use
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