Short Description
Quantifies Akt (S473) phosphorylation in whole cells using an indirect ELISA method.
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ Human/Mouse AKT1(S473) Phosphorylation ELISA Kit offers a high-throughput solution for monitoring the PI3K/Akt signaling pathway by quantifying pAKT1(S473) levels directly in cultured cells. By integrating total protein normalization within the same well, this cell-based system eliminates lysate preparation and minimizes experimental variability, facilitating precise screening of kinase inhibitors and activators in drug discovery.
Features
High-Precision Detection: This assay ensures exceptional sensitivity and specificity for phosphorylated Serine 473, minimizing cross-reactivity with non-phosphorylated Akt isoforms.
Streamlined Workflow: The high-throughput microplate format includes pre-coated surfaces and optimized reagents to reduce hands-on time and experimental variability.
Robust Reproducibility: Rigorous quality control guarantees consistent performance across different batches, providing reliable quantitative data for longitudinal research studies.
Species Versatility: Validated for both human and murine cell lysates, allowing for seamless transition between clinical models and preclinical animal studies.
Process Relevance
Quantifying Akt activation to evaluate kinase inhibitor efficacy and characterize therapeutic modulation of survival-related signaling cascades.
Application Stage
Drug discovery, preclinical candidate validation, and molecular mechanism-of-action profiling.
Applications
Ideal for cell-based screening, kinase assays, and monitoring AKT1 activation in response to growth factors or pharmacological inhibitors using various mammalian cell lysates.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Specifically detects phosphorylated human or mouse AKT1 at the serine 473 residue.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 453 nm)
Research Areas
Oncology therapeutics, metabolic disorders, immunology, neurobiology, regenerative medicine, etc.
Datasheet