Short Description
Quantifies GSK3β phosphorylation at Serine 9 in cell lysates using a sandwich ELISA method.
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ Human/Mouse GSK3Β(S9) Phosphorylation ELISA Kit provides a rapid and sensitive solution for quantifying phosphorylated GSK3β (Ser9) in cultured cells. This high-throughput compatible system eliminates cell lysis requirements by utilizing in-situ fixation and normalization to total protein. It is an essential tool for screening pathway modulators and optimizing drug candidates in metabolic and neurological research.
Features
Superior Sensitivity: Employs high-affinity antibody pairs to detect low-abundance phosphorylated GSK3β with exceptional precision and a broad linear dynamic range.
Dual-Species Cross-Reactivity: Validated for high performance in both human and murine cell lysates, facilitating seamless transitions between clinical and preclinical models.
Robust Assay Format: Streamlined sandwich ELISA protocol ensures high reproducibility and minimizes hands-on time while maintaining rigorous experimental consistency.
Process Relevance
Quantifying GSK3β Ser9 phosphorylation enables precise monitoring of kinase inhibition during therapeutic screening and pathway modulation assays.
Application Stage
Discovery-stage drug screening, lead optimization, and molecular mechanism validation in academic and industrial laboratory settings.
Applications
In vitro kinase assays, high-throughput inhibitor screening, and characterization of PI3K/Akt pathway activation across diverse cancerous or neurodegenerative cell populations.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Specifically quantifies GSK3β protein levels phosphorylated at the regulatory serine 9 residue.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 455 nm)
Research Areas
Metabolic syndromes, oncology therapeutics, neurobiology, cell signaling, stem cell pluripotency, etc.
Datasheet