SigPath™ Human/Mouse GSK3Β(S9) Phosphorylation ELISA Kit

CAT#: ITS-1125-WB9
Product Type: Assay Kit
Species: Human, mouse, rat
Target: Specifically quantifies GSK3β protein levels phosphorylated at the regulatory serine 9 residue.
Brand: SigPath
Development Stage: Research-Grade Signal Pathway Assay Development
Short Description
Quantifies GSK3β phosphorylation at Serine 9 in cell lysates using a sandwich ELISA method.
Brand
SigPath
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ Human/Mouse GSK3Β(S9) Phosphorylation ELISA Kit provides a rapid and sensitive solution for quantifying phosphorylated GSK3β (Ser9) in cultured cells. This high-throughput compatible system eliminates cell lysis requirements by utilizing in-situ fixation and normalization to total protein. It is an essential tool for screening pathway modulators and optimizing drug candidates in metabolic and neurological research.
Features
Superior Sensitivity: Employs high-affinity antibody pairs to detect low-abundance phosphorylated GSK3β with exceptional precision and a broad linear dynamic range.
Dual-Species Cross-Reactivity: Validated for high performance in both human and murine cell lysates, facilitating seamless transitions between clinical and preclinical models.
Robust Assay Format: Streamlined sandwich ELISA protocol ensures high reproducibility and minimizes hands-on time while maintaining rigorous experimental consistency.
Process Relevance
Quantifying GSK3β Ser9 phosphorylation enables precise monitoring of kinase inhibition during therapeutic screening and pathway modulation assays.
Application Stage
Discovery-stage drug screening, lead optimization, and molecular mechanism validation in academic and industrial laboratory settings.
Applications
In vitro kinase assays, high-throughput inhibitor screening, and characterization of PI3K/Akt pathway activation across diverse cancerous or neurodegenerative cell populations.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Specifically quantifies GSK3β protein levels phosphorylated at the regulatory serine 9 residue.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 455 nm)
Sample Type
Cells
Research Areas
Metabolic syndromes, oncology therapeutics, neurobiology, cell signaling, stem cell pluripotency, etc.
Size
100 tests
Data Output Type
Quantitative protein phosphorylation measurement (endpoint absorbance at 450 nm).
Assay Time
6.5 h
Species
Human, mouse, rat
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
GSK3β remains constitutively active until inhibitory Ser9 phosphorylation occurs via the Akt pathway. This modification governs glucose homeostasis, microtubule stability, and the survival-to-apoptosis transition in diverse cellular environments.
Pathways
The Ser9 modification is primarily mediated by upstream PI3K/Akt signaling. Downstream, inhibited GSK3β allows for the stabilization of β-catenin and the activation of glycogen synthase for energy storage.
For Research Use Only | Not For Clinical Use
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