Targeted Protein Degradation Service
Introduction Strategies Workflow What We Can Offer Why Choose Us FAQs Customer Reviews Extended Services Contact
Revolutionizing Drug Discovery: Advanced Targeted Protein Degradation Assay Service
Targeted Protein Degradation (TPD) represents a revolutionary strategy in drug discovery, moving beyond traditional protein inhibition to achieve complete protein removal. By hijacking the cell's natural Ubiquitin-Proteasome System (UPS), TPD offers a powerful approach to target previously "undruggable" proteins. This catalytic mechanism leads to superior pharmacodynamics, overcoming drug resistance and expanding the therapeutic landscape. TPD Assay Service at Creative Biolabs provides the critical tools and expertise to explore this innovative modality, accelerating the identification and development of novel therapeutics.
Are you currently facing long drug development cycles, difficulty in identifying novel therapeutic targets, or challenges in overcoming drug resistance? Our Targeted Protein Degradation Assay Service helps you accelerate drug discovery, unlock novel therapeutic avenues, and overcome resistance through advanced TPD platforms, high-throughput screening, and comprehensive mechanism-of-action elucidation. Our service is designed to streamline your research by providing robust assays for ternary complex formation, target engagement, and quantitative protein degradation, ensuring you obtain reliable results for lead candidate selection and optimization.
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Strategies of This Service
Target-Centric vs. Ligase-Centric Approaches
We adapt our strategy based on your project's focus. For novel targets, we can screen against a panel of diverse E3 ligases to identify optimal ligase-target pairs. Alternatively, if you have a specific E3 ligase of interest, we can tailor assays to identify degraders that recruit it to your target.
High-Throughput Screening (HTS) Optimization
Our HTS strategies are optimized for efficiency and data quality. We utilize miniaturized assay formats and automated liquid handling systems to screen large compound libraries rapidly, identifying primary hits with high confidence.
Mechanism of Action (MoA) Elucidation
Beyond simple degradation measurement, we prioritize understanding the precise MoA. This includes detailed studies of ternary complex formation, target engagement within cells, and direct ubiquitination of the target protein, providing critical insights for rational drug design.
Cellular and Biochemical Assay Integration
We integrate both cellular and biochemical assays to provide a holistic view of degrader activity. Biochemical assays offer precise control over components, while cellular assays confirm activity in a physiologically relevant environment, ensuring translational relevance.
Structure-Activity Relationship (SAR) Support
Our data generation and analysis are designed to support SAR studies, helping you understand how chemical modifications impact degrader potency, selectivity, and mechanism, thereby guiding lead optimization.
Workflow
Our meticulously designed workflow ensures a systematic and efficient progression of your TPD projects, providing clear outcomes at each stage. This process is fully transparent and can be visualized as a clear flowchart for your convenience.
Project Consultation & Design
We begin with an in-depth discussion to understand your specific project goals, target protein characteristics, and desired outcomes. Based on this, our experts design a customized assay strategy, selecting appropriate E3 ligases, assay formats, and detection technologies to ensure optimal results.
Assay Development & Optimization
Our team develops and rigorously optimizes the chosen TPD assays (e.g., ternary complex formation, cellular degradation, ubiquitination assays) for your specific target and compounds. This includes establishing optimal concentrations, incubation times, and detection methods to ensure assay robustness and sensitivity.
High-Throughput Screening (HTS)
Utilizing our state-of-the-art automated platforms, we conduct high-throughput screening of your compound libraries against the optimized TPD assays. This allows for rapid identification of potent and selective degrader candidates.
Mechanism of Action Elucidation
For promising hits, we perform in-depth mechanistic studies, including quantitative analysis of ternary complex formation, target engagement, and direct ubiquitination assays. This step confirms the on-target mechanism of degradation.
Data Analysis & Reporting
All raw data undergoes rigorous quality control and comprehensive analysis by our bioinformatics specialists. You receive detailed reports summarizing assay results, hit identification, potency data, and mechanistic insights, presented in an easily digestible format.
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Required Starting Materials: To initiate your project, clients typically provide:
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Target Protein Information: Details on your protein of interest (POI), including its sequence, known binding sites, and any available recombinant protein or cell line expressing the target.
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Small Molecule Compounds: Your library of potential degrader molecules for screening.
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E3 Ligase Details: Information on the specific E3 ubiquitin ligase you intend to recruit, if known, or guidance on preferred ligases for screening.
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Final Deliverables: Upon project completion, you will receive:
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Comprehensive Data Reports: Detailed reports including raw data, analyzed results, dose-response curves, and statistical analyses for all assays performed.
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Lead Compound Profiles: Characterization data for identified degrader hits, including potency, selectivity, and mechanistic insights.
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Expert Recommendations: Strategic recommendations for further compound optimization and progression based on the generated data.
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Estimated Timeframe: The typical timeframe for our targeted protein degradation assay service ranges from 8 to 16 weeks, depending on the complexity of the target, the size of the compound library, and the scope of mechanistic studies required. Custom projects may have varying durations, which will be clearly communicated during the initial consultation.
What We Can Offer
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E3 Ligase & Target Identification: We provide access to a diverse panel of validated E3 ubiquitin ligases and offer robust services for target validation and degradability assessment, helping you identify suitable proteins for TPD.
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Degrader Design & Synthesis Support: Our experts offer rational design strategies for PROTACs and molecular glues, considering optimal E3 ligase and target binding, linker chemistry, and physicochemical properties. We also support custom synthesis and focused library generation.
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Mechanism of Action & Biophysical Characterization: We perform critical assays for ternary complex formation using advanced technologies. We also conduct target engagement assays and comprehensive biophysical analyses for binding kinetics and thermodynamics.
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Cellular Degradation Assays: We offer quantitative protein degradation measurement using high-throughput assays. We also confirm target ubiquitination and assess downstream phenotypic effects.
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In Vitro & In Efficacy Studies: Our services include comprehensive potency and selectivity profiling, pharmacokinetic (PK) and pharmacodynamic (PD) studies, and preclinical validation of degrader efficacy in relevant disease models.
Why Choose Us?
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Industry-Leading Expertise
Choosing Creative Biolabs means partnering with a trusted leader in targeted protein degradation, backed by deep scientific knowledge and a proven track record in drug discovery.
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Experienced Scientific Team
Our team includes veteran scientists with decades of combined experience in biochemistry, molecular biology, and medicinal chemistry, specializing in the complexities of protein degradation.
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Cutting-Edge Technology
We leverage state-of-the-art automated screening platforms and advanced analytical tools to generate precise, reproducible, and high-quality data.
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Integrated End-to-End Workflow
Our comprehensive services span from initial target assessment to detailed mechanistic studies, ensuring a seamless and efficient degrader discovery process.
Experience the Creative Biolabs Advantage - Get a Quote Today.
FAQs
What types of targets can be addressed with your TPD assay service?
Our platform is versatile and can address a wide range of targets, including those considered "undruggable" by traditional small molecules. We encourage you to discuss your specific target with our scientific team to assess its suitability for TPD.
How do you ensure the selectivity of the degraders identified?
We employ rigorous selectivity profiling assays across a panel of related and unrelated proteins to ensure that the identified degraders are highly specific to your target of interest, minimizing off-target effects.
Can you help with both PROTACs and molecular glues?
Yes, our expertise and assay capabilities extend to both PROTACs and molecular glues. We can design and execute assays tailored to either class of degrader, supporting your specific drug discovery approach.
What kind of data will I receive at the end of the service?
You will receive comprehensive data reports including raw data, analyzed results, dose-response curves, statistical analyses, lead compound profiles, and expert recommendations for further optimization.
Do you offer support for in vivo studies?
Yes, our platform includes capabilities for pharmacokinetic (PK) and pharmacodynamic (PD) studies, as well as efficacy validation in relevant animal models, providing crucial data for preclinical development.
What if my target protein is difficult to express or purify?
Our team has extensive experience with challenging proteins. We can assist with strategies for protein expression and purification or adapt cellular assay formats to circumvent these challenges, ensuring your project can proceed effectively.
Customer Reviews
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"Exceptional Potency Data! Using Targeted Protein Degradation Assay Service at Creative Biolabs in our research has significantly improved our ability to identify highly potent degraders. The detailed reports and mechanistic insights were invaluable for our lead optimization efforts." - Dr. J. Smi**, Lead Scientist, Pharma R&D, 2024
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"Streamlined Workflow! The comprehensive platform at Creative Biolabs greatly facilitated our early-stage PROTAC discovery. Their expertise in E3 ligase selection and assay development saved us considerable time and resources compared to in-house efforts." - Prof. A. Bro**, Academic Researcher, University of Biosciences, 2023
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"Reliable & Reproducible Results! We've consistently relied on Creative Biolabs for our cellular degradation assays. The consistency and reproducibility of their data are critical for our decision-making, allowing us to confidently advance our most promising compounds." - Ms. K. Cha**, Senior Biologist, Biotech Startup, 2025
Extended Services
Beyond our core Targeted Protein Degradation Assay Service, Creative Biolabs offers a range of complementary services and sub-options to support your broader drug discovery and development goals:
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Protein Production & Engineering: High-quality recombinant protein expression and purification services, essential for biochemical TPD assays and structural studies.
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Antibody Discovery & Development: Services for generating highly specific antibodies, which can be crucial for Western Blot analysis in TPD studies or for developing antibody-PROTAC conjugates.
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Cell Line Development: Custom cell line generation, including stable cell lines expressing your target protein or specific E3 ligases, for robust cellular TPD assays.
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Structural Biology Services: X-ray crystallography and cryo-EM services to elucidate the 3D structures of target proteins, E3 ligases, and ternary complexes, providing crucial insights for rational degrader design.
Contact Creative Biolabs Today
The future of drug discovery is here, driven by targeted protein degradation. At Creative Biolabs, we are uniquely positioned to help you navigate this exciting frontier and translate groundbreaking science into life-changing medicines. Our comprehensive targeted protein degradation assay service provides the expertise, technology, and insights you need to identify, validate, and optimize novel degrader therapeutics.
Contact Our Team for More Information and to Discuss Your Project.
