NF-AT Transcription Factor

Nuclear Factor of Activated T-cells (NF-AT) is a pivotal transcription factor family essential for the regulation of gene expression in response to immune stimuli, particularly within T cells. This family comprises several members, including NF-ATc1, NF-ATc2, NF-ATc3, and NF-ATc4, which play significant roles in T cell activation, differentiation, and the overall immune response. Upon dephosphorylation by the phosphatase calcineurin in response to elevated intracellular calcium levels, NF-AT translocates to the nucleus, where it influences the expression of various genes involved in immune responses, inflammation, and cell growth. NF-AT proteins are also implicated in various physiological processes and pathological conditions, such as transplant rejection, autoimmune diseases, and cancer. Given its importance in immune modulation, NF-AT has become a focal point for therapeutic research, with significant efforts directed towards the identification of agonists and antagonists that can modulate its activity.

Innovative NF-AT Transcription Response Assay Service

At Creative Biolabs, we are proud to offer top-tier transcription response assay services tailored to screen and quantify the functional activity of test compounds as either agonists or antagonists against human NF-AT. Our NF-AT Transcription Response Assay is meticulously designed to provide researchers with precise and reliable data, facilitating the advancement of novel therapeutic agents targeting NF-AT-related pathways. Utilizing human T lymphocytic Jurkat cells transfected with a response element-lacZ reporter, where the transcription of the β-galactosidase gene is controlled by the NF-AT-1 transcription factor binding site, our assay provides precise and reliable measurements of NF-AT-mediated transcriptional activation or inhibition. This enables researchers to comprehensively evaluate the impact of potential therapeutic agents, thereby advancing drug discovery and development projects aimed at modulating immune responses.

Transcription Factor Assay Principle

Our assay employs human T lymphocytic Jurkat cells, which are transfected with a response element-lacZ reporter construct. In this system, the transcription of the β-galactosidase gene is controlled by the binding site for the NF-AT-1 transcription factor. Upon activation, NF-AT binds to the response element, driving the expression of β-galactosidase. By utilizing a fluorescein di-β-d-galactopyranoside (FDG)-based substrate, the expression of β-galactosidase can be assessed and subsequently quantified to serve as an indicator of NF-AT activity.

Applications

Accelerate Your Research with Creative Biolabs

• High Sensitivity and Specificity: Our assay employs advanced detection technologies to ensure high sensitivity and specificity, accurately measuring even minute changes in NF-AT activity.
• High Throughput Capability: The assay is designed for high-throughput screening, allowing the evaluation of numerous samples in a single run. This efficiency makes it highly suitable for large-scale drug discovery and development projects.
• Robust and Reproducible Results: With stringent controls and validated protocols, we deliver reliable and reproducible data, minimizing variability and enhancing confidence in your research findings.
• Comprehensive Data Analysis: Our service includes detailed data analysis, presenting clear and actionable insights into the transcriptional response elicited by your test compounds.

For specific project needs or detailed inquiries, please contact us, we are committed to offering bespoke solutions tailored to your research goals.

For Research Use Only | Not For Clinical Use