The binding assay is conducted by exposing sensors with serially diluted antibody clones in the binding buffer in wells of a 96-well microtiter plate. Binding kinetics are measured using an advanced sensor and transformation system.
B Cell based Biolayer Interferometry (BLI) Assay Service
Creative Biolabs' B Cell-based Bio-layer interferometry assay is a comprehensive solution designed to address the most critical bottlenecks in antibody and therapeutic protein development. We deliver not just data, but actionable insights that guide your project from early-stage screening to lead candidate selection. Clients can expect a complete characterization of binding kinetics, affinity, and specificity, enabling them to make informed decisions and accelerate their pipeline.
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The Bio-layer interferometry (BLI) Technique
The bio-layer interferometry (BLI) biosensor system detects and analyzes the interference pattern of white light reflected from the plate where proteins are immobilized. In the BLI assay, the biomolecules in a sample can bind to the sensor plate and cause shifts in light reflection and interference patterns. Moreover, only protein binding or dissociation can change the interference pattern. Other parameters, such as refractive index, pH, and other matrix effects, do not influence the response profile. The changes are recorded in real-time and transferred to visual curves, enabling fast measurement of binding affinity, association, and dissociation kinetics of antibody-antigen interactions.
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Fig.1 Schematics of bio-layer interferometry strategy for the identification of active viral epitope.1
What We Can Offer
BLI Assay Arrangement and Data Process
Service Deliverables
We deliver binding curves and key kinetic data, including association rate (kon), dissociation rate (koff), and the equilibrium dissociation constant (KD). We also provide qualitative binding analysis.
BLI Assay Services at Creative Biolabs
Highlights
Label-Free Analysis
Our BLI platform is a label-free technology, saving valuable time on sample preparation. It also avoids potential signal interference, ensuring that the binding data you receive is a true reflection of the molecular interaction.
Real-Time Data
We capture and document dynamic interactions as they happen, providing a complete picture of your specific binding events. This real-time data is critical for confident decision-making throughout your discovery pipeline.
High Throughput
Our system is capable of screening 96 samples in a single experiment, enabling rapid optimization of binding conditions. You can screen thousands of samples in hours, significantly accelerating your research timeline.
Actionable Insights
Beyond raw data, our expert team provides in-depth analysis and interpretation. We transform complex sensorgrams into clear, actionable insights, helping you select the most promising lead candidates for your project.
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Customer Reviews
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Unmatched Speed and Efficiency
Using Creative Biolabs' BLI assay has significantly improved our ability to rapidly identify and characterize lead candidates. The label-free format and high-throughput capabilities allowed us to process more samples in a fraction of the time. - Jane P****. -
A Reliable Partner.
We were concerned about the complexity of our project, but the team at Creative Biolabs provided clear guidance and a reliable workflow. Their BLI assay enabled us to confidently select a highly specific antibody for our functional studies, proving to be a highly effective alternative to SPR for our needs. - Sarah R*****.
FAQs
Can your assay handle difficult-to-purify antibodies or crude samples?
Yes, one of the primary benefits of our BLI assay is its compatibility with complex sample matrices, such as cell lysates and hybridoma supernatants. This eliminates the need for extensive purification, saving you significant time and resources without compromising data quality.
What types of interactions can be analyzed with your BLI assay?
Our platform is optimized for a wide range of interactions, including antibody-antigen, protein-protein, and protein-small molecule binding. It provides comprehensive kinetic and affinity data for all these applications.
Related Services
T and B Cell-based Surface Plasmon Resonance (SPR) Assay
Utilize our SPR assay for an alternative, fluidics-based method to measure real-time binding kinetics, affinity, and concentration. It is ideal for a wide range of molecular interactions.
Learn More →T and B Cell-based X-ray Crystallography Assay
Obtain high-resolution 3D structures of your antibodies and antigens to understand their precise binding mechanisms, aiding in rational drug design and optimization.
Learn More →How to Contact Us
With the advanced biolayer interferometry technology platform established for years and scientists experienced in cancer antigen discovery, Creative Biolabs provides incomparable BLI services to meet all your demands. We promise reliable, accurate, specific, reproducible, and high-quality BLI services for clients. Please contact us to get more information on the BLI assay.
Reference
- Petersen, Rejane L. "Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development." Biosensors vol. 7,4 49. 31 Oct. 2017. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/bios7040049
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