Biosensor Technology

To modernize the endotoxin detection method, Creative Biolabs provides biosensor technology to detect the presence or absence of endotoxin in various test samples. This technology includes three main categories: electrochemical technology, optical technology, mass-based technology.

Three Categories

• Electrochemical biosensor

Majority of electrochemical biosensors are based on electrochemical impedance spectroscopy (EIS) which is performed in the solution with electrodes. The electrodes are coated in metal, to reduce electric resistance. These electrodes bind to endotoxin neutralizing proteins (ENPs), which have highly selective to endotoxin components. This means that if endotoxins come in contact with the electrode-protein complex, they bind to the proteins. Therefore, the electrochemical biosensor has the ability to distinguish and detect endotoxin from a mixture of proteins, nucleic acids and phospholipids.

• Optical biosensor

Multiple optical biosensors have successfully been developed for the endotoxin assay:

• Bioluminescence-based optical biosensor is the endotoxin assay using mutant firefly luciferase, which is based on the same principle of the LAL assay except for the endpoint material (pNA).
• Fluorescence-based optical biosensor is an endotoxin detection strategy with a low endotoxin detection limit and high selectivity.
• Liquid crystal (LC)-based optical biosensor, a highly sensitive endotoxin detection technology, is developed using endotoxin-specific single-stranded DNA aptamers.
• Surface plasmon resonance (SPR)-based optical biosensor is a developing biosensor platform, which utilized the smartphone's built-in flash as a light source and a compact diffraction grating and spectra dispersive unit.
• Mass-based biosensor
• Electromagnetic piezoelectric acoustic sensing (EMPAS) and Electromagnetic piezoelectric acoustic sensors are the real-time endotoxin detection assay, which is based on an ultrathin, oligoethylene glycol-based mixed surface platform coated on piezoelectric quartz discs.
• Magnetoelasticity is another mass-based method that functions by placing sensors directly on dry testing surfaces.

Features of Biosensor Technology

• Fast response
• Easy operation
• High sensitivity
• High selectivity
• Great stability

Creative Biolabs has been committed to the research and development of multiple strategies for endotoxin detection. We provide all the above technologies to help you meet all your detection needs. Moreover, with the improvement of other materials, such as microfabrication and nanomaterials, we will continue to develop integrated and miniaturized endotoxin detection devices with high sensitivity and great stability in the future. For more detailed information, please feel free to contact us or directly send us an inquiry.

References

1. Dullah, E.C.; et al. Current trends in endotoxin detection and analysis of endotoxin-protein interactions. Crit Rev Biotechnol. 2017, 37(2): 251-261.
2. Schneier, M.; et al. Current technologies to endotoxin detection and removal for biopharmaceutical purification. Biotechnology and Bioengineering. 2020, 117(8): 2588-2609.

For Research Use Only | Not For Clinical Use