Cell Migration Assay Services
Driving Innovation in Cellular Movement
Cell migration plays a critical role in numerous biological processes, from tissue development and wound healing to immune surveillance and cancer metastasis. Understanding the mechanisms of cell movement is essential for advancing research in fields such as oncology, regenerative medicine, and immunology.
At Creative Biolabs, we are proud to introduce our specialized cell migration assay services, designed to facilitate cutting-edge research by providing precise, high-throughput solutions for studying cellular motility. Among these services, our Epidermal Growth Factor (EGF)-induced HaCaT Cell Migration Assay stands out. This assay is particularly tailored for research involving keratinocyte behavior, making it a powerful tool for studying wound healing, skin diseases, and epithelial cell biology. Using Epidermal Growth Factor (EGF) to stimulate the migration of HaCaT cells, this assay offers key insights into cellular dynamics under physiologically relevant conditions.
Technical Methods
Creative Biolabs employs an array of advanced techniques to conduct cell migration assays, offering a versatile and thorough approach to studying cellular movement.
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Assays
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Description
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Wound Healing (Scratch) Assay
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A traditional method to study collective cell migration. Cells are cultured to form a monolayer, and a "wound" is made by scratching the surface. The migration of cells into the wound area is then monitored over time.
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Transwell Migration Assay
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This method quantifies cell migration through a porous membrane in response to chemotactic agents. It's ideal for evaluating the effects of chemoattractants or inhibitors on cell migration.
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Chemotaxis Assay
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Measures directional migration of cells in response to chemical gradients. This technique is highly useful for studying immune cell migration or cancer cell invasion.
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Live-Cell Imaging
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Allows real-time visualization of cell movement, providing dynamic data and enabling precise tracking of migration patterns over time.
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High-Throughput Screening
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Facilitates the simultaneous testing of multiple conditions or compounds to identify factors that influence cell migration, enhancing the efficiency of drug discovery and biological studies.
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Publication Sharing
This study reveals that cell migration can be effectively inhibited by cytochalasin D and mitomycin C. Cytochalasin D, an inhibitor of actin polymerization, disrupts the cytoskeletal structure, resulting in a marked reduction in cell migration. Mitomycin C, known for inhibiting DNA synthesis, primarily blocks cell proliferation but also contributes to decreased migration by preventing the involvement of newly divided cells. The findings underscore the roles of the cytoskeleton and proliferation in cell migration, demonstrating how these two factors influence migratory behavior through distinct yet interconnected pathways.
Fig.1 Cell migration is inhibited by cytochalasin D and mitomycin C.1
Advantages
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Customizable assays: Our assays are fully customizable, allowing you to choose specific cell types, growth factors, or experimental conditions based on your research objectives. Whether you're investigating cancer cell migration, immune cell chemotaxis, or epithelial wound healing, we tailor the assay to your needs.
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Enhanced data accuracy: Our integration of real-time live-cell imaging provides detailed, dynamic insights into cellular migration patterns, giving you access to more comprehensive data compared to static endpoint assays.
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EGF-specific insights: The EGF-induced HaCaT cell migration assay provides targeted information on how epidermal cells respond to EGF stimulation. This is especially valuable for researchers focusing on skin biology, including wound repair, skin diseases, and epithelial regeneration.
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High-throughput capabilities: Our high-throughput platforms allow for the screening of multiple conditions, making our migration assays suitable for large-scale studies or compound screening, helping to accelerate your research timeline.
If you're seeking to explore the intricacies of cell migration or require a focused study on cell migration, Creative Biolabs is here to provide you with cutting-edge solutions tailored to your research needs. Our expert team is dedicated to delivering precise, reliable, and customizable assays that drive innovation and discovery in the field of cellular migration. Contact us today to discuss how we can assist you in advancing your research with our comprehensive Cell Migration Assay Services.
Reference
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Glenn, Honor L., Jacob Messner, and Deirdre R. Meldrum. "A simple non-perturbing cell migration assay insensitive to proliferation effects." Scientific Reports 6.1 (2016): 31694. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only | Not For Clinical Use
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