Short Description
Quantifies target protein expression in cultured cells using a colorimetric cell-based ELISA method.
Development Stage
Research-Grade Cell-Based Assay Development
Description
The CelluTest™ Cell-Based Custom ELISA Kit provides a rapid, sensitive platform for quantifying target proteins directly in cultured cells without the need for lysate preparation. This high-throughput compatible system enables precise monitoring of signaling pathways and drug effects, using total protein normalization to minimize well-to-well variation for reliable pre-clinical screening.
Features
Homogeneous High-Throughput Format: Employs a "mix-and-read" protocol that eliminates tedious wash steps and cell lysis, significantly reducing hands-on time.
Superior Dynamic Sensitivity: Detects subtle changes in protein levels with high signal-to-noise ratios, ensuring precision across diverse experimental conditions.
Physiologically Relevant Data: Analyzes targets within the native intracellular context, preserving protein-protein interactions and post-translational modifications often lost in biochemical assays.
Flexible Modular Components: Provides optimized reagents and specific enzyme-tagged vectors tailored for seamless integration into various mammalian cell lines.
Process Relevance
Enabling real-time monitoring of intracellular protein dynamics to optimize lead candidate selection and ensure pharmacological consistency.
Application Stage
Ideal for hit-to-lead screening, lead optimization, preclinical potency validation, and rigorous in-process quality control.
Applications
Quantifying protein degradation, translocation, and secretion across oncology, immunology, and neurology models using high-throughput screening of small molecules or therapeutic biologics.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Intracellular protein concentrations, subcellular localization, and dynamic expression profiles in mammalian cells.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 450 nm)
Research Areas
Oncology, drug discovery, signal transduction, metabolic diseases, immunology, etc.
