CytoFinal™ Pig Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit

CAT#: ITS-0326-WB69
Product Type: ELISA Kit
Species: Pig
Target: Pig alanine aminotransferase 1 (ALT/GPT)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively detects pig alanine aminotransferase 1 (ALT/GPT) protein levels using a sensitive ELISA-based assay.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Pig Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit provides a reliable immunoassay for quantitative measurement of ALT1 protein in porcine biological samples. Using a sandwich ELISA format with highly specific antibodies, the kit enables accurate detection of ALT levels in serum, plasma, tissue lysates, and cell culture supernatants, supporting liver function studies and preclinical toxicology research.
Features
High-affinity antibody pair enables selective recognition of porcine ALT with excellent specificity and minimal interference from homologous enzymes.
Sensitive sandwich ELISA format supports accurate quantification of low-abundance ALT protein in complex biological matrices.
Standard 96-well microplate configuration facilitates efficient processing of multiple samples using widely available ELISA plate readers.
Carefully optimized reagents and calibration standards provide consistent signal generation and reproducible quantitative results across independent experiments.
Process Relevance
Quantifies ALT protein levels to evaluate liver injury and metabolic enzyme regulation in porcine experimental models.
Application Stage
Preclinical research, toxicology studies, veterinary biomarker analysis, and translational liver disease research.
Applications
Quantitative measurement of porcine ALT protein in serum, plasma, tissue homogenates, and cell culture samples for hepatotoxicity studies, metabolic research, biomarker evaluation, ADME profiling studies, and veterinary investigations.
Target
Pig alanine aminotransferase 1 (ALT/GPT)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Veterinary medicine, hepatology, toxicology, metabolic disease research, pharmacokinetics (ADME), pharmacology, translational biomedical research, etc.
Size
96 tests
Data Output Type
Quantitative measurement of ALT protein concentration based on absorbance signals generated in a sandwich ELISA assay.
Assay Range
4.69 ng/ml - 300 ng/ml
Species
Pig
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Alanine aminotransferase (ALT), also known as glutamate-pyruvate transaminase, is a cytosolic enzyme primarily expressed in hepatocytes. It catalyzes the transfer of amino groups between alanine and α-ketoglutarate, producing pyruvate and glutamate. Elevated ALT levels in biological samples often indicate hepatocellular damage or metabolic imbalance.
Pathways
ALT participates in the alanine-glucose cycle, linking peripheral amino acid metabolism with hepatic gluconeogenesis. The pyruvate produced feeds into the tricarboxylic acid (TCA) cycle and gluconeogenic pathways, integrating nitrogen metabolism with cellular energy production.
For Research Use Only | Not For Clinical Use
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