CytoFinal™ Cow Lactate Dehydrogenase B (LDHB) ELISA Kit
CAT#: ITS-0326-WB121
Product Type: ELISA Kit
Species: Cow
Target: Lactate Dehydrogenase B (LDHB)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies cow LDHB concentrations in tissues, cells, and biological fluids using sensitive ELISA methodology.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Cow Lactate Dehydrogenase B (LDHB) ELISA Kit provides a precise in vitro solution for quantifying LDHB levels in tissue homogenates, cell lysates, and other biological fluids, supporting metabolic and veterinary research.
Features
High specificity and sensitivity for cow LDHB detection, minimizing cross-reactivity with other lactate dehydrogenase isoforms. Colorimetric sandwich ELISA format with pre-coated 96-well plates ensures consistent, reproducible quantification. Broad detection range (0.78-50 ng/mL) allows accurate measurement across diverse sample types. Stable lyophilized standards and reagents maintain activity for six months under recommended storage conditions.
Process Relevance
Monitors LDHB levels to support metabolic profiling and enzyme activity assessment in cow-derived samples.
Application Stage
Drug discovery, veterinary diagnostics, and biochemical research.
Applications
Quantitative analysis of LDHB in tissue homogenates, cell lysates, serum, or plasma samples for ADME profiling , enzymatic, or biomarker studies.
Target
Lactate Dehydrogenase B (LDHB)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Quantitative measurement of enzyme concentration using colorimetric endpoint detection.
Assay Range
0.78 ng/ml - 50 ng/ml
Species
Cow
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
LDHB catalyzes the reversible conversion of lactate to pyruvate, critical for energy metabolism in mammalian tissues. Measuring its levels informs studies on metabolic activity, tissue health, and disease states in cattle.
Pathways
LDHB participates in glycolysis and gluconeogenesis pathways, linking lactate-pyruvate interconversion. Alterations influence cellular energy balance, redox homeostasis, and lactate-driven metabolic flux.