CytoFinal™ Standard Rat Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit

CAT#: ITS-0326-WB91
Product Type: ELISA Kit
Species: Rat
Target: Rat cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures cytoplasmic AST (GOT1) levels in rat serum, plasma, tissues, and cell lysates with high reliability.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Standard Rat AST (GOT1) ELISA Kit provides a robust, in vitro method to detect cytoplasmic AST across diverse rat biological samples. It supports preclinical research, hepatology studies, and metabolic investigations with reproducible results in serum, plasma, tissues, and cell lysates.
Features
Utilizes a high-specificity sandwich ELISA format to accurately detect cytoplasmic AST in multiple rat sample types.
Provides sensitive measurement with a broad dynamic range, ensuring precise quantification under various experimental conditions.
Compatible with serum, plasma, tissue homogenates, and cell lysates, offering versatile use in preclinical research applications.
Includes standardized reagents and lyophilized controls for reproducible, long-term performance across multiple assay runs.
Process Relevance
Enables accurate monitoring of cytoplasmic AST to assess liver function and metabolic activity in rat preclinical studies.
Application Stage
Applicable in preclinical research, hepatology studies, and laboratory-based quality control processes.
Applications
Quantitative detection of AST in rat serum, plasma, tissues, and cell lysates for metabolic studies, biomarker discovery, ADME profiling studies, and hepatotoxicity evaluation.
Target
Rat cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatant and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, metabolic disease research, enzymology, biomarker development, pharmacokinetics (ADME), preclinical pharmacology, translational research, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of cytoplasmic AST activity in rat biological samples.
Assay Range
0.78 ng/ml - 50 ng/ml
LOD
< 0.39 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
AST/GOT1 catalyzes the reversible transamination of aspartate and α-ketoglutarate, crucial for amino acid metabolism and energy production. Its cytoplasmic levels reflect hepatocellular integrity and metabolic function in rat models.
Pathways
AST participates in the malate-aspartate shuttle, regulating cytoplasmic-mitochondrial redox balance. Activity is influenced by amino acid availability, supporting TCA cycle flux and overall energy metabolism.
For Research Use Only | Not For Clinical Use
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