CytoFinal™ Human Lactate Dehydrogenase B (LDHB) ELISA Kit

CAT#: ITS-0326-WB116
Product Type: ELISA Kit
Species: Human
Target: Human Lactate Dehydrogenase B (LDHB)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitative ELISA kit for measuring human Lactate Dehydrogenase B (LDHB) levels in biological samples.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Human Lactate Dehydrogenase B (LDHB) ELISA Kit provides a sensitive immunoassay for the quantitative detection of LDHB protein in human-derived samples. Based on a high-performance sandwich ELISA with HRP-driven colorimetric detection, this kit enables reliable measurement of LDHB in serum, plasma, tissue lysates, and cultured cells for metabolic and disease-related studies.
Features
Highly specific sandwich ELISA employing validated antibody pairs enables precise detection of human LDHB with minimal cross-reactivity to related LDH isoforms.
Optimized assay sensitivity and broad dynamic range ensure reliable quantification of LDHB across diverse biological sample matrices.
HRP-TMB colorimetric detection generates stable absorbance signals compatible with standard microplate readers for efficient and reproducible data acquisition.
Comprehensive kit configuration including precoated microplates, standards, and optimized buffers simplifies experimental workflows and enhances assay reproducibility.
Process Relevance
Measures LDHB levels to evaluate metabolic balance and oxidative energy metabolism in cellular and disease models.
Application Stage
Clinical biomarker research, metabolic pathway studies, drug discovery, and translational biomedical research.
Applications
Quantification of human LDHB in serum, plasma, tissue lysates, and cultured cells for metabolic pathway studies, cardiac research, cytotoxicity analysis, ADME profiling studies, and biomarker discovery.
Target
Human Lactate Dehydrogenase B (LDHB)
Detection Method
Colorimetric
Sample Type
Serum, plasma and other biological fluids.
Assay Measures
Quantitative
Research Areas
Cardiovascular biology, cancer metabolism, metabolic disease research, pharmacokinetics (ADME), biomarker discovery, toxicology, cellular metabolism, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of LDHB protein concentration determined by ELISA absorbance.
Assay Range
0.78 ng/ml - 50 ng/ml
LOD
0.47 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase B (LDHB) is a major isoform of the LDH enzyme family that is predominantly expressed in oxidative tissues such as heart and brain. Unlike LDHA, LDHB preferentially catalyzes the conversion of lactate into pyruvate, supporting aerobic metabolism. Altered LDHB expression has been associated with metabolic reprogramming and several disease conditions.
Pathways
LDHB plays an important role in the glycolysis-gluconeogenesis metabolic network by converting lactate to pyruvate while generating NADH. This reaction links cytosolic lactate metabolism to mitochondrial oxidative phosphorylation. LDHB activity therefore contributes to cellular energy homeostasis and metabolic adaptation in highly oxidative tissues.
For Research Use Only | Not For Clinical Use
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