CytoFinal™ High-Sensitivity Human Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit

CAT#: ITS-0326-WB88
Product Type: ELISA Kit
Species: Human
Target: Human cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures human cytoplasmic AST (GOT1) levels in serum, plasma, tissues, and cell lysates with high sensitivity.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ High-Sensitivity Human AST (GOT1) ELISA Kit provides a robust in vitro solution for detecting cytoplasmic AST concentrations in various biological fluids. The kit enables precise, reproducible quantification, supporting biomarker analysis and disease research.
Features
Employs a sandwich ELISA format with pre-coated antibodies for sensitive, specific detection of human cytoplasmic AST across diverse samples.
High assay sensitivity (0.19 ng/mL) with broad detection range (0.31-20 ng/mL) for accurate quantification.
Compatible with serum, plasma, tissue homogenates, cell lysates, and other biological fluids, enabling versatile experimental applications.
Lyophilized standards and stable HRP-conjugated reagents ensure consistent performance and long-term storage under controlled conditions.
Process Relevance
Monitors cytoplasmic AST levels to assess hepatocellular integrity and metabolic activity in research and clinical studies.
Application Stage
Applicable in biomarker validation, drug discovery, disease modeling, and in-process laboratory quality control.
Applications
Quantitative assessment of AST activity in serum, plasma, tissues, and cell lysates, enabling metabolic profiling, hepatology studies, ADME profiling studies, and biomarker validation.
Target
Human cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Enzymology, hepatology, tumor immunology, infection immunity, metabolic disease research, pharmacokinetics (ADME), biomarker development, etc.
Size
96 tests
Data Output Type
Quantitative measurement of cytoplasmic AST concentration using colorimetric readout.
Assay Range
0.31 ng/ml - 20 ng/ml
LOD
0.19 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Aspartate Aminotransferase (AST/GOT1) is a key cytoplasmic enzyme in amino acid metabolism, catalyzing reversible conversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate. Its levels reflect hepatocellular health and metabolic flux, making it a vital biomarker for liver function and systemic metabolic studies.
Pathways
AST (GOT1) participates in the malate-aspartate shuttle, linking cytoplasmic and mitochondrial redox states. Upstream, it responds to amino acid availability; downstream, it affects the tricarboxylic acid (TCA) cycle and energy metabolism.
For Research Use Only | Not For Clinical Use
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