CytoFinal™ Standard Human Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit

CAT#: ITS-0326-WB78
Product Type: ELISA Kit
Species: Human
Target: Human alanine aminotransferase 1 (ALT1/GPT)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Sensitive ELISA kit for detecting and quantifying human ALT1 protein in serum, plasma, tissues, and cell lysates.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Standard Human Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit enables accurate, in vitro measurement of ALT1 levels in human biological samples. Using a high-affinity antibody sandwich format, this kit allows reliable evaluation of hepatocellular integrity, liver function, and metabolic enzyme activity in clinical and research settings.
Features
High specificity: Detects ALT1 with minimal cross-reactivity to other aminotransferase isoforms.
Reliable sensitivity: Measures ALT1 across a broad concentration range in human samples.
Convenient 96-well microplate format: Supports high-throughput assays and standard ELISA readers.
Comprehensive reagents included: Ready-to-use standards, buffers, and detection components ensure reproducible results.
Process Relevance
Provides quantitative assessment of ALT1 to monitor liver function and detect hepatocellular stress in human samples.
Application Stage
Clinical research, translational studies, biomarker analysis, and preclinical liver evaluation workflows.
Applications
Measures ALT1 in human serum, plasma, tissue, and cell lysates for hepatotoxicity studies, liver disease monitoring, ADME profiling studies, and metabolic pathway investigations.
Target
Human alanine aminotransferase 1 (ALT1/GPT)
Detection Method
Colorimetric
Sample Type
Serum, plasma, cell culture supernatants, tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, metabolic disease research, pharmacology, pharmacokinetics (ADME), translational medicine, biomarker discovery, preclinical liver safety evaluation, etc.
Size
96 tests
Data Output Type
Quantitative measurement of ALT1 protein levels via absorbance-based sandwich ELISA readouts.
Assay Range
1.56 ng/ml - 100 ng/ml
LOD
< 0.63 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
ALT1 catalyzes the reversible conversion of alanine and α-ketoglutarate into pyruvate and glutamate, linking amino acid metabolism with gluconeogenesis. Blood and tissue ALT1 levels reflect liver function, hepatocellular integrity, and metabolic homeostasis.
Pathways
ALT1 participates in the alanine cycle, connecting amino acid catabolism to hepatic gluconeogenesis. Pyruvate produced can feed into the TCA cycle, integrating nitrogen metabolism with energy production and cellular homeostasis.
For Research Use Only | Not For Clinical Use
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