CytoFinal™ High-Sensitivity Human Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit

CAT#: ITS-0326-WB77
Product Type: ELISA Kit
Species: Human
Target: Human alanine aminotransferase 1 (ALT1/GPT)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
High-sensitivity ELISA kit for precise quantification of ALT1 (GPT) protein levels in human biological samples.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ High-Sensitivity Human Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit provides a robust, in vitro solution for measuring ALT1 protein in human serum, plasma, tissues, and cell lysates. Leveraging a sandwich ELISA format with highly specific antibodies, this kit enables accurate monitoring of liver function, hepatocellular stress, and metabolic enzyme dynamics in clinical and translational research.
Features
Human-specific ALT1 antibodies ensure high selectivity, minimizing cross-reactivity with related aminotransferase isoforms.
Enhanced sensitivity detects low-abundance ALT1 in complex human biological matrices.
96-well microplate format supports high-throughput analysis compatible with standard laboratory ELISA readers.
Complete reagents and calibrated standards enable reproducible results with minimal experimental variation.
Process Relevance
Quantifies ALT1 levels in human samples to assess liver health, hepatocellular injury, and metabolic function.
Application Stage
Clinical research, translational studies, biomarker discovery, and preclinical liver function evaluation.
Applications
Measures ALT1 in human serum, plasma, tissues, and cultured cells for liver function studies, hepatotoxicity assessment, ADME profiling studies, and metabolic pathway analysis.
Target
Human alanine aminotransferase 1 (ALT1/GPT)
Detection Method
Colorimetric
Sample Type
Serum, plasma, cell culture supernatants, tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Clinical hepatology, translational liver research, metabolic disease studies, pharmacology, pharmacokinetics (ADME), preclinical biomarker evaluation, drug-induced liver injury monitoring, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using absorbance-based sandwich ELISA methodology.
Assay Range
0.312 ng/ml - 20 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
ALT1 catalyzes reversible transamination between alanine and α-ketoglutarate, forming pyruvate and glutamate. Its levels in human blood and tissues reflect hepatocellular integrity and metabolic homeostasis, making it a key biomarker for liver function.
Pathways
ALT1 participates in the alanine cycle, linking amino acid metabolism to hepatic gluconeogenesis. Pyruvate generated enters the TCA cycle or gluconeogenic pathways, connecting nitrogen metabolism to energy production in liver physiology.
For Research Use Only | Not For Clinical Use
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