PharmaOnco™ CellSort Two-Step Mouse Treg (CD4+CD25+) Cell Selection Kit, 10 tests

CAT#: ITS-1222-YT40
Product Type: Cell Isolation Kit
Species: Mouse
Description
This kit has been developed to isolate highly purified mouse Treg cells ( CD25+CD4+) from mouse spleen by immunomagnetic negative selection and the followed positive selection. Isolated cells ​are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and magnetic beads; Desired cells are labeled with antibodies and magnetic beads.
Comment
This kit is available for processing 1 x 10^9 cells.
Method Type
Negative selection; positive selection
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Other, Spleen
Size
1 kit, 10 tests (∼1x10^8 cells/test)
Components
PharmaOnco™ Mouse CD4+ T Cell Cocktail, 1 mL
PharmaOnco™ Streptavidin nanobeadsMouse Biotinylated CD25 antibody, 1 mL
PharmaOnco™ Streptavidin Nanoparticles, 1.5 mL
PharmaOnco™ Buffer, 25 mL
Material not included
Magnetic separator
Cell Background
Regulatory T (Treg) cells
Species
Mouse
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Handling Advice
Please place the product at proper storage condition once received.
Storage
Store at 2 - 8°C.
Do not freeze.
For Research Use Only | Not For Clinical Use
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