PharmaOnco™ CHO-Tg(Human LIGHT) Stable Cell Line, Single Clone

Creative Biolabs offers CHO-Tg(Human LIGHT) stable cell line which the LIGHT gene stably expressed in CHO cells.

CHO-Tg(Human LIGHT) stable cell line is a cell line derived from Chinese Hamster Ovary (CHO) cells that have been transfected with a gene encoding human LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with herpes simplex virus glycoprotein D for HVEM, a receptor expressed by T lymphocytes). This cell line overexpresses human LIGHT, allowing research on the biological functions and signaling pathways of this protein.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

LIGHT

Human

CHO

Genbank #NM_003807

The LIGHT gene, also known as TNFSF14, is a protein-coding gene that encodes a cytokine belonging to the tumor necrosis factor (TNF) ligand family. It plays a crucial role in the immune response by activating various signaling pathways involved in inflammation, cell survival, and immune cell activation. The LIGHT protein binds to its receptor, HVEM (herpesvirus entry mediator), and acts as a co-stimulatory molecule in T-cell activation and proliferation. It also interacts with other receptors such as LTβR (lymphotoxin beta receptor) and DcR3 (decoy receptor 3), thereby influencing immune cell function. Dysregulation of the LIGHT gene has been implicated in various autoimmune diseases, chronic inflammatory conditions, and cancer. Targeting the LIGHT signaling pathway has emerged as a potential therapeutic strategy for these conditions.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.