ModifiSite™ Aldehyde Dehydrogenase Inhibitor Screening Kit

CAT#: ITS-1125-WB36
Product Type: Assay Kit
Species: All Species
Target: Aldehyde dehydrogenase (ALDH) superfamily proteins including human isoforms ALDH1A1, ALDH2, and ALDH3A1.
Short Description
Identifies ALDH inhibitors by measuring enzyme activity via a NADH-coupled fluorometric method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Aldehyde Dehydrogenase Inhibitor Screening Kit provides a rapid, high-throughput solution for identifying ALDH modulators in drug discovery. This homogeneous, "mix-incubate-measure" system is essential for screening compounds targeting alcohol metabolism and cancer stem cells. By offering a reliable non-radioactive platform, it supports lead optimization and the development of therapies for alcoholism and cancer.
Features
Enhanced Sensitivity: Employs a high-efficiency diaphorase-coupled colorimetric system for precise quantification of enzymatic activity and subtle inhibitory effects.
HTS-Ready Format: The mix-incubate-measure workflow is optimized for 96-well plates and seamlessly integrates with automated liquid handling platforms.
Rapid Turnaround: Delivers stable, reproducible kinetic or endpoint data in less than one hour with minimal hands-on reagent handling.
Exceptional Specificity: Tailored assay chemistry minimizes background interference, ensuring high-confidence screening of chemical libraries against the ALDH superfamily.
Process Relevance
Optimizing inhibitor potency and selectivity to accelerate lead candidate transitions from primary screening to preclinical drug development.
Application Stage
Target validation, high-throughput compound library screening, and lead optimization during early-stage medicinal chemistry and drug discovery.
Applications
Evaluation of small molecule modulators, IC50 determination, and enzyme kinetics analysis across diverse biological samples, supporting oncological research and metabolic disorder drug development.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Aldehyde dehydrogenase (ALDH) superfamily proteins including human isoforms ALDH1A1, ALDH2, and ALDH3A1.
Detection Method
Absorbance at 565 nm
Sample Type
Chemical compounds
Research Areas
Oncology therapeutics, pharmacokinetics (ADME), metabolic diseases, toxicology, stem cell biology, etc.
Size
100 tests
Data Output Type
Quantitative enzyme inhibition measurements via absorbance-based kinetic or endpoint assays at 565 nm.
Assay Time
30 min
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Aldehyde dehydrogenases are critical oxidoreductases that neutralize toxic aldehydes, protecting cells from oxidative stress. Their dysregulation is a hallmark of alcohol sensitivity, neurodegeneration, and malignant transformation.
Pathways
ALDH enzymes are central to ethanol metabolism, retinoic acid signaling, and reactive oxygen species (ROS) detoxification. They regulate downstream lipid peroxidation products and upstream carboxylic acid biosynthesis for homeostasis.
For Research Use Only | Not For Clinical Use
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