Short Description
Identifies and characterizes NOS inhibitors by measuring nitric oxide production using a colorimetric method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Nitric Oxide Synthase Inhibitor Screening Kit provides a rapid, non-radioactive platform for evaluating NOS modulators in drug discovery. This high-throughput compatible system utilizes an improved Griess method to measure NO production, supporting the development of therapeutic candidates for cardiovascular and neurological disorders through precise inhibition profiling and reliable kinetic data.
Features
High Sensitivity: Detects enzyme activity at levels as low as 0.25 U/L using advanced non-radioactive colorimetric signaling methods.
Automation Ready: Optimized "mix-incubate-measure" format ensures seamless integration with standard HTS liquid handling systems and 96-well microplates.
Rapid Workflow: Complete characterization of potential inhibitors in under three hours, significantly accelerating hit-to-lead discovery and drug development timelines.
Robust Specificity: Specifically designed to evaluate multiple NOS isoforms (nNOS, eNOS, iNOS) with minimal interference from complex sample matrices.
Process Relevance
Streamlining the identification of small-molecule modulators to assess therapeutic potency and isoform selectivity during enzymatic process optimization.
Application Stage
Preclinical drug discovery, lead optimization, and high-throughput screening for pharmaceutical development and in-process quality assessment.
Applications
Screening chemical libraries for potential inhibitors, characterizing compound IC50 values, and evaluating NOS modulators in cardiovascular, immunological, and neurodegenerative disease research models.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Nitric oxide synthase isoforms (nNOS, iNOS, and eNOS) and their enzymatic conversion of L-arginine.
Detection Method
Absorbance at 540 nm
Sample Type
Nitric oxide synthase
Research Areas
Cardiovascular therapeutics, immunology & inflammation, neuropharmacology, oncology, vascular physiology, etc.
Datasheet