ModifiSite™ Nitric Oxide Synthase Inhibitor Screening Kit

CAT#: ITS-1125-WB38
Product Type: Assay Kit
Species: All Species
Target: Nitric oxide synthase isoforms (nNOS, iNOS, and eNOS) and their enzymatic conversion of L-arginine.
Short Description
Identifies and characterizes NOS inhibitors by measuring nitric oxide production using a colorimetric method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Nitric Oxide Synthase Inhibitor Screening Kit provides a rapid, non-radioactive platform for evaluating NOS modulators in drug discovery. This high-throughput compatible system utilizes an improved Griess method to measure NO production, supporting the development of therapeutic candidates for cardiovascular and neurological disorders through precise inhibition profiling and reliable kinetic data.
Features
High Sensitivity: Detects enzyme activity at levels as low as 0.25 U/L using advanced non-radioactive colorimetric signaling methods.
Automation Ready: Optimized "mix-incubate-measure" format ensures seamless integration with standard HTS liquid handling systems and 96-well microplates.
Rapid Workflow: Complete characterization of potential inhibitors in under three hours, significantly accelerating hit-to-lead discovery and drug development timelines.
Robust Specificity: Specifically designed to evaluate multiple NOS isoforms (nNOS, eNOS, iNOS) with minimal interference from complex sample matrices.
Process Relevance
Streamlining the identification of small-molecule modulators to assess therapeutic potency and isoform selectivity during enzymatic process optimization.
Application Stage
Preclinical drug discovery, lead optimization, and high-throughput screening for pharmaceutical development and in-process quality assessment.
Applications
Screening chemical libraries for potential inhibitors, characterizing compound IC50 values, and evaluating NOS modulators in cardiovascular, immunological, and neurodegenerative disease research models.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Nitric oxide synthase isoforms (nNOS, iNOS, and eNOS) and their enzymatic conversion of L-arginine.
Detection Method
Absorbance at 540 nm
Sample Type
Nitric oxide synthase
Research Areas
Cardiovascular therapeutics, immunology & inflammation, neuropharmacology, oncology, vascular physiology, etc.
Size
100 tests
Data Output Type
Quantitative measurement of enzyme inhibition percentage based on kinetic or endpoint absorbance at 540 nm.
Assay Time
2-3 h
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Nitric oxide synthase (NOS) catalyzes the production of nitric oxide, a critical gaseous signaling molecule. While physiologically essential, dysregulated NOS activity is a hallmark of hypertension, septic shock, and chronic inflammation.
Pathways
Involved in the L-arginine-NO pathway, where NOS produces NO. This assay converts NO to nitrite for Griess colorimetric detection at 540 nm, enabling efficient screening of potential NOS inhibitors.
For Research Use Only | Not For Clinical Use
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