Short Description
Quantifies trypsin inhibitor activity using a fluorometric (or fluorescence polarization) method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Trypsin Inhibitor Assay Kit provides a high-throughput, fluorescence-based platform for screening potential drug candidates and protease inhibitors. Utilizing a quenched FITC-labeled substrate, this homogeneous "mix-incubate-measure" system offers superior sensitivity and a robust Z' factor (>0.7). Its rapid 45-minute workflow enables efficient characterization of inhibitor potency and selectivity in pharmaceutical development.
Features
Rapid Analytical Throughput: Utilizes a streamlined 96-well microplate format to achieve reliable quantitative results within a concise 20-minute incubation period.
Optimized Detection Precision: Engineered for high sensitivity using a synthetic substrate, ensuring minimal sample volume requirements while maintaining robust signal-to-noise ratios.
Broad Sample Compatibility: Versatile assay chemistry accommodates diverse biological matrices including plant extracts, food products, serum, and purified protein fractions.
Convenient Mix-and-Read Protocol: Features stable, pre-formulated reagents that simplify complex enzymatic workflows into a user-friendly, non-radioactive detection procedure.
Process Relevance
Optimizing protease inhibition profiles to ensure enzymatic stability and nutritional quality across pharmaceutical and food processing workflows.
Application Stage
Lead compound screening, bio-process optimization, and rigorous in-process quality control for therapeutic protein production.
Applications
Quantitative evaluation of inhibitor potency in plant seeds, serum screenings, food safety testing, and high-throughput identification of synthetic serine protease inhibitors.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Inhibitory molecules targeting the serine protease trypsin.
Detection Method
Fluorescence Detection or Fluorescence Polarization (Ex 485 nm / Em 530 nm)
Sample Type
Chemical compounds
Research Areas
Nutritional science, gastroenterology, drug discovery (protease inhibitors), seed physiology, and toxicology.
Datasheet