OXPHOSight™ Phospholipid Assay Kit

CAT#: ITS-1125-YL19
Product Type: Assay Kit
Species: All Species
Short Description
Measures phospholipids using a colorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Phospholipid Assay Kit offers a sensitive colorimetric method for quantifying total phospholipids in biological samples such as serum and cell lysates. Utilizing an enzymatic digestion and oxidation process, the system produces a colored complex proportional to the choline concentration. This analytical tool is vital for researchers investigating lipid metabolism, membrane composition, and signaling pathways, providing high accuracy for fundamental studies in cell biology.
Features
Targets choline-containing phospholipids via a specific enzymatic cascade, facilitating rapid lipid profiling and membrane composition analysis in various cell-based and tissue research models.
Process Relevance
Assessing total phospholipid content to monitor membrane integrity and lipid metabolic flux in vitro.
Application Stage
Lipidomics, membrane research, and metabolic profiling.
Applications
Quantify total phospholipids in serum and tissue homogenates to evaluate lipid metabolism and cellular membrane composition during research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, plasma, tissue homogenates, cell lysates.
Research Areas
Lipidomics; Membrane Biology; Metabolic Research
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
20 min
LOD
LOD: OD, FL: 3, 0.6 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
These amphiphilic molecules are the primary components of cellular membranes and play essential roles in cell signaling and lipid metabolism. Monitoring total phospholipid levels is crucial for understanding membrane dynamics and lipid-related physiological processes.
Pathways
Phospholipids are fundamental components of cellular membranes and precursors for signaling molecules like diacylglycerol (DAG) and inositol trisphosphate (IP3). They are involved in downstream signaling of G-protein coupled receptors and receptor tyrosine kinases.
For Research Use Only | Not For Clinical Use
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