Short Description
Measures phospholipids using a colorimetric method.
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Phospholipid Assay Kit offers a sensitive colorimetric method for quantifying total phospholipids in biological samples such as serum and cell lysates. Utilizing an enzymatic digestion and oxidation process, the system produces a colored complex proportional to the choline concentration. This analytical tool is vital for researchers investigating lipid metabolism, membrane composition, and signaling pathways, providing high accuracy for fundamental studies in cell biology.
Features
Targets choline-containing phospholipids via a specific enzymatic cascade, facilitating rapid lipid profiling and membrane composition analysis in various cell-based and tissue research models.
Process Relevance
Assessing total phospholipid content to monitor membrane integrity and lipid metabolic flux in vitro.
Application Stage
Lipidomics, membrane research, and metabolic profiling.
Applications
Quantify total phospholipids in serum and tissue homogenates to evaluate lipid metabolism and cellular membrane composition during research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, plasma, tissue homogenates, cell lysates.
Research Areas
Lipidomics; Membrane Biology; Metabolic Research
Datasheet