T Cell based BrdU Assay Service
Are you currently facing challenges in accurately and reliably measuring T cell proliferation for your immuno-oncology projects? Creative Biolabs' T cell-based BrdU assay service helps you accelerate drug discovery and therapeutic development by providing a precise and non-radioactive method for assessing the functional competence of T cells. The assay quantifies new DNA synthesis, directly reflecting proliferative capacity, which is crucial for evaluating immuno-oncology drug candidates and understanding the strength of an anti-tumor immune response.
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BrdU: The Modern Alternative to Outdated Methods
Bromo-2'-deoxyuridine (BrdU), also known as bromodeoxyuridine, is an analog of thymidine. T cell proliferation is a fundamental biological process essential for a successful adaptive immune response. The T cell-based BrdU assay is a powerful method for quantifying this process based on DNA synthesis. It provides a highly specific and sensitive readout of cellular division by measuring the incorporation of BrdU, a thymidine analog, into new DNA strands during the S-phase of the cell cycle. This approach has become the modern standard in immunology and immuno-oncology, replacing older, hazardous radioactive methods. Cited literature supports the efficacy and safety of this technology, confirming its use as a reliable tool for studying T cell activation and therapeutic effects.
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T Cell-based BrdU Assay Services at Creative Biolabs
The T cell-based BrdU assay workflow at Creative Biolabs is a comprehensive and streamlined process designed for maximum clarity and efficiency, ensuring that your research project is executed with precision.
Why Choose Us?
Choosing our T cell-based BrdU assay brings a host of distinct advantages to your drug discovery and development pipeline:
High Sensitivity and Precision
Our assays deliver reliable and reproducible data, which is critical for making informed decisions during preclinical research and clinical translation.
Accelerated Drug Screening
Rapidly assess the anti-proliferative or agonistic effects of your compounds on T cells, helping you to identify and optimize lead candidates with greater efficiency.
Comprehensive Insights
Go beyond simple cell counts. The BrdU assay provides a direct measurement of DNA synthesis, offering a deeper mechanistic understanding of how your compound affects cell cycle progression.
Cost-Effective Solutions
By outsourcing these complex and time-consuming assays to Creative Biolabs, you can optimize your resource allocation and focus on your core research and development.
For more information or to discuss how our assays can accelerate your immuno-oncology project, please do not hesitate to contact our expert team.
FAQs
Q1: Can I use the T cell-based BrdU assay for high-throughput screening?
A1: Yes. Our assay is highly scalable and can be performed in multi-well plate formats, making it ideal for high-throughput screening of large compound libraries. We can design a customized workflow to meet your specific throughput needs.
Q2: Does the BrdU assay affect cell function or viability?
A2: BrdU itself is generally non-toxic at the concentrations used in our protocols. While the standard BrdU detection process requires a DNA denaturation step that can be harsh, our optimized protocols minimize any potential impact on cell viability and morphology.
Q3: What kind of controls are included in the assay?
A3: We include a series of rigorous controls in every assay. These typically include unstimulated cells as a negative control, and a mitogen (e.g., PHA or anti-CD3/CD28 antibodies) as a positive control for T cell proliferation.
Customer Review
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Efficiency and Safety
Using Creative Biolabs' assay in our research has significantly improved the efficiency of our in vitro toxicity studies. We've been able to move away from our outdated radioactive assays, saving us time and ensuring safer lab conditions. - J. S*ith
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Comparative Analysis
We used their service for a comparative analysis of our lead compounds. The ability to use a non-radioactive assay like BrdU, combined with their flow cytometry expertise, gave us a clear picture of our compound's effect on cell proliferation without the cytotoxicity we've observed with some dilution-based dyes. - R. M**gan
Related Services
To help you achieve your project goals, Creative Biolabs offers a range of complementary services and related options:
T Cell Activation Analysis
Creative Biolabs provides optimal and customizable T-cell activation analysis solutions. Our flexible, multi-method approach quickly identifies T-cell activation status, advancing your research with tailored experimental support and practical insights.
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Cytokine Production Measurement Service
Creative Biolabs offers comprehensive cytokine production measurement services for diverse research needs. Our skilled scientists analyze dysregulated cytokines in the tumor microenvironment, providing customized solutions and in-depth insights for clients.
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Contact Us
Creative Biolabs is dedicated to providing the precise data that fuels the next generation of cancer medicines. Our T cell-based BrdU assay is more than a service; it is a partnership that empowers you to gain clear, actionable insights into immune responses and therapeutic efficacy. Contact us today to discuss how we can accelerate your immuno-oncology project.
Reference
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Stempels, F. C., et al. "A sensitive and less cytotoxic assay for identification of proliferating T cells based on bioorthogonally-functionalized uridine analogue." Journal of immunological methods 502 (2022): 113228. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.1016/j.jim.2022.113228
For Research Use Only | Not For Clinical Use
