Vesitra™ Human ENT2 Stable HEK293 Cell Line

CAT#: ITS-0326-HM24
Product Type: Transporter Cell Line
Organism: Human
Brand: Vesitra™
Development Stage: ADME
ADME Research Domain: Metabolism; Absorption; Excretion
Short Description
An ENT2 stable expression model focused on nucleoside uptake research in muscle and specialized tissues.
Brand
Vesitra™
Development Stage
ADME
ADME Research Domain
Metabolism; Absorption; Excretion
Description
Compared to ENT1, ENT2 possesses a more unique transport capacity for nucleobases. This cell line aims to help researchers distinguish the varying contributions of ENT isoforms in complex tissues, particularly in nucleoside salvage and gene therapy vector delivery studies.
Features
1. Validated unique nucleobase transport activity. 2. Designed to pair with the ENT1-only expression model to achieve subtype-specific differentiation. 3. Robust cell growth kinetics adaptable for large-scale 384-well plate screening.
Applications
Studying the uptake of nucleotide metabolic drugs in muscle tissues; Developing specialized nucleoside analogs targeting ENT2.
Parental Cell Line
Human Embryonic Kidney 293 (HEK293)
Transfection
Stable Transfection
Transporter
ENT2
Gene
SLC29A2
Typical Probe Substrate
Adenosine / Uridine
Reference Inhibitor
Dipyridamole (NBMPR-insensitive)
Organism
Human
Strain Name
HEK293
Tissue
Embryonic Kidney
Background
ENT2 (SLC29A2) shares a similar bidirectional transport profile with ENT1 but exhibits a broader substrate specificity that includes nucleobases. It plays a significant role in the salvage pathway of nucleosides and is a vital cellular entry point for specific nucleobase analog therapeutics in various tissues, including skeletal muscle.
Morphology
Epithelial
Unit Size
2×10⁶ / vial lyophilized cells
Culture Properties
Adherent
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
Online Inquiry