Vesitra™ Human CNT2 Stable HEK293 Cell Line

CAT#: ITS-0326-HM26
Product Type: Transporter Cell Line
Organism: Human
Brand: Vesitra™
Development Stage: ADME
ADME Research Domain: Metabolism; Absorption; Excretion
Short Description
A CNT2 model dedicated to purine nucleoside active transport research, decoding energy-dependent uptake mechanisms.
Brand
Vesitra™
Development Stage
ADME
ADME Research Domain
Metabolism; Absorption; Excretion
Description
This is a sodium-dependent transport system optimized specifically for purine nucleosides. The Vesitra™ CNT2 cell line provides a highly sensitive and clean-background environment for studying the transmembrane kinetics of adenosine analogs and drugs like ribavirin.
Features
1. Stable energy-driven uptake assay window. 2. Verified specificity using adenosine and guanosine substrates. 3. Excellent cell health conditions supporting automated liquid handling operations.
Applications
Screening nucleoside prodrugs with tissue-targeting properties; Investigating hepatic and intestinal retrieval of purine metabolites.
Parental Cell Line
Human Embryonic Kidney 293 (HEK293)
Transfection
Stable Transfection
Transporter
CNT2
Gene
SLC28A2
Typical Probe Substrate
Adenosine / Inosine
Reference Inhibitor
Phlorizin
Organism
Human
Strain Name
HEK293
Tissue
Embryonic Kidney
Background
CNT2 (SLC28A2) is a sodium-coupled active transporter specifically tailored for the high-affinity uptake of purine nucleosides (such as adenosine and inosine). Its specialized physiological role makes it an important mechanism for the cellular salvage of purines and the targeted delivery of purine analog therapeutics.
Morphology
Epithelial
Unit Size
2×10⁶ / vial lyophilized cells
Culture Properties
Adherent
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
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