Vesitra™ Human OAT3 Stable HEK293 Cell Line

CAT#: ITS-0326-HM7
Product Type: Transporter Cell Line
Organism: Human
Brand: Vesitra™
Development Stage: ADME
ADME Research Domain: Metabolism; Absorption; Excretion
Short Description
A comprehensive OAT3 model for decoding renal anion secretion, preferred for screening macromolecules and conjugated metabolites.
Brand
Vesitra™
Development Stage
ADME
ADME Research Domain
Metabolism; Absorption; Excretion
Description
Optimized for handling anionic substrates with higher lipophilicity or larger molecular weights, this model sensitively captures uptake activities missed by OAT1. It provides indispensable in vitro parameters for building complete PBPK models of renal excretion.
Features
1. Exceptional substrate affinity with high sensitivity to clinical markers like Estrone-3-sulfate (E3S). 2. Robust morphology maintaining a compact cobblestone shape post-passage for stable membrane protein retention.
Applications
Evaluation of renal excretion for phase II metabolites (e.g., glucuronide conjugates); Renal OAT family DDI screening.
Parental Cell Line
Human Embryonic Kidney 293 (HEK293)
Transfection
Stable Transfection
Transporter
OAT3
Gene
SLC22A8
Typical Probe Substrate
Estrone-3-sulfate (E3S) / Benzylpenicillin
Reference Inhibitor
Probenecid / Novobiocin
Organism
Human
Strain Name
HEK293
Tissue
Embryonic Kidney
Background
OAT3 (SLC22A8), alongside OAT1, is localized to the basolateral membrane of the kidney's proximal tubules. It exhibits a slightly broader substrate specificity than OAT1, handling larger, more lipophilic organic anions and conjugates (e.g., estrone-3-sulfate, benzylpenicillin). OAT3 screening is a vital component of robust DDI risk assessments.
Morphology
Epithelial
Unit Size
2×10⁶ / vial lyophilized cells
Culture Properties
Adherent
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
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