Vesitra™ Human Mock HEK293 Cell Line

CAT#: ITS-0326-HM45
Product Type: Transporter Cell Line
Organism: Human
Brand: Vesitra™
Development Stage: ADME
ADME Research Domain: Metabolism; Absorption; Excretion
Short Description
The essential negative control cell line transfected with an empty vector, required for determining background transport activity.
Brand
Vesitra™
Development Stage
ADME
ADME Research Domain
Metabolism; Absorption; Excretion
Description
The Vesitra™ Human Mock HEK293 Cell Line undergoes the exact same transfection and antibiotic selection process as our target transporter cell lines, but with an empty expression vector. It serves as the definitive negative control, allowing researchers to accurately subtract endogenous background noise and calculate the specific signal-to-noise ratio for any target transporter assay.
Features
1. Genetically matched background to all Vesitra™ HEK293 transporter models. 2. Cultured and selected under identical antibiotic pressure for true comparability. 3. Essential for calculating net uptake/efflux ratios in regulatory ADME filings.
Applications
Establishing baseline cellular uptake/efflux; Validating assay specificity; Calculating specific transport activity by subtracting background noise.
Parental Cell Line
Human Embryonic Kidney 293 (HEK293)
Transfection
Stable Transfection
Transporter
Control
Organism
Human
Strain Name
HEK293
Tissue
Embryonic Kidney
Background
Accurate transporter characterization requires isolating specific active transport from passive diffusion. The Mock HEK293 cell line is an isogenic control transfected with an empty vector. It serves as the mandatory physiological baseline for all uptake assays, ensuring background transport can be subtracted to calculate precise net uptake and signal-to-noise ratios.
Morphology
Epithelial
Unit Size
2×10⁶ / vial lyophilized cells
Culture Properties
Adherent
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
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