Vesitra™ Human OCT2 Stable HEK293 Cell Line

CAT#: ITS-0326-HM9
Product Type: Transporter Cell Line
Organism: Human
Brand: Vesitra™
Development Stage: ADME
ADME Research Domain: Metabolism; Absorption; Excretion
Short Description
A fully ICH-compliant OCT2 model unlocking the key window into renal tubular secretion and accumulation of cationic drugs.
Brand
Vesitra™
Development Stage
ADME
ADME Research Domain
Metabolism; Absorption; Excretion
Description
Passing multi-dimensional functional validation, our Vesitra™ Human OCT2 cell line strongly emphasizes uptake responses to chemotherapeutics and histamine antagonists. It helps pharmaceutical companies accurately identify competitive inhibition in the kidneys pre-clinically, reducing late-stage DDI failures.
Features
1. Gold-standard bi-directional validation using specific markers like MPP+ and Cimetidine for every batch. 2. Extended functional lifespan maintaining peak transport activity within recommended passages post-thaw.
Applications
Acute Kidney Injury (AKI) mechanism assessment for platinum-based chemotherapeutics (e.g., cisplatin); DDI filings for renal cationic clearance pathways.
Parental Cell Line
Human Embryonic Kidney 293 (HEK293)
Transfection
Stable Transfection
Transporter
OCT2
Gene
SLC22A2
Typical Probe Substrate
MPP+ / Metformin
Reference Inhibitor
Cimetidine / Quinidine
Organism
Human
Strain Name
HEK293
Tissue
Embryonic Kidney
Background
OCT2 (SLC22A2) is the primary cation uptake transporter in the kidney, situated on the basolateral membrane of proximal tubules. It facilitates the entry of circulating basic drugs from the blood into renal cells prior to their urinary excretion. Given its central role in renal clearance, OCT2 evaluation is critical in early preclinical drug assessment.
Morphology
Epithelial
Unit Size
2×10⁶ / vial lyophilized cells
Culture Properties
Adherent
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
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