Vesitra™ Human OCTN2 Stable HEK293 Cell Line

CAT#: ITS-0326-HM17
Product Type: Transporter Cell Line
Organism: Human
Brand: Vesitra™
Development Stage: ADME
ADME Research Domain: Metabolism; Absorption; Excretion
Short Description
The benchmark OCTN2 stable cell line for studying carnitine cycling and drug-induced efflux blockade.
Brand
Vesitra™
Development Stage
ADME
ADME Research Domain
Metabolism; Absorption; Excretion
Description
This is a highly sodium-dependent carnitine transport model. Vesitra™ OCTN2 precisely simulates carnitine uptake in the kidneys and muscles, serving as an authoritative platform for evaluating the risk of secondary carnitine deficiency induced by drug candidates.
Features
1. Strict sodium-dependency validation yielding an excellent signal-to-noise ratio. 2. Dual validation covering both carnitine and diverse organic cation substrates. 3. Meets in vitro characterization requirements for OCTN pathways in regulatory DDI filings.
Applications
Screening carnitine analogs or competitive inhibitors; Assessing the impact of antiepileptic drugs (e.g., valproic acid) on carnitine homeostasis.
Parental Cell Line
Human Embryonic Kidney 293 (HEK293)
Transfection
Stable Transfection
Transporter
OCTN2
Gene
SLC22A5
Typical Probe Substrate
L-Carnitine
Reference Inhibitor
Quinidine / Verapamil
Organism
Human
Strain Name
HEK293
Tissue
Embryonic Kidney
Background
OCTN2 (SLC22A5) functions both as an organic cation transporter and a high-affinity, sodium-dependent symporter for L-carnitine. Its primary physiological role is maintaining systemic carnitine homeostasis, which is essential for fatty acid oxidation. Drugs that inhibit OCTN2 can lead to secondary carnitine deficiency and associated toxicities.
Morphology
Epithelial
Unit Size
2×10⁶ / vial lyophilized cells
Culture Properties
Adherent
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
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