Mammalian Cell Glycoengineering Services

Mammalian Cell Glycoengineering for Therapeutic Glycoprotein Production

Mammalian cells such as Chinese hamster ovary (CHO) cells have been extensively utilized in the biopharmaceutical industry as the production hosts for recombinant protein therapeutics, especially glycoproteins, due to several key advantages such as scalability, high production rates, and human-like glycosylation. Currently, the majority of therapeutic biologics are manufactured using the CHO cell lines. There are differences in glycosylation between human and nonhuman mammalian cells. In particular, there are two nonhuman glycans, α1,3-Gal and Neu5Gc, that exist in mouse cells. In addition, the glycosylation patterns of proteins expressed in CHO and human cell lines are likely to differ significantly. CHO cells typically do not express GnT-III, resulting in the absence of bisecting GlcNAc residues in their glycoforms. These differences in glycosylation can impact the bioactivity of glycoproteins produced in CHO cells, particularly therapeutic biologics. Therefore, glycoengineering in nonhuman mammalian cells is indispensable for the production of recombinant human glycoproteins.

Fig.1 Therapeutic glycoproteins produced in CHO cells.¹

Mammalian Cell Glycoengineering Services at Creative Biolabs

Creative Biolabs has achieved significant success in diverse Cell Line Glycoengineering, including the CHO and other nonhuman mammalian cell lines, for the production of human glycoproteins. Our achievements encompass both N-linked and O-linked glycoengineering. To obtain customized glycoprotein products across various applications, we perform tailored glycoengineering strategies, such as reducing glycan heterogeneity to ensure consistent glycan profiles, and other modifications to improve the biological activity of glycoprotein biologics. We provide various glycoengineering strategies and services encompassing genetic engineering, metabolic engineering, and in vitro enzymatic engineering based on the specific needs of our clients.

Fig.2 Strategies for glycoengineering in mammalian cells. (Creative Biolabs Original)

Combined with advanced engineering techniques, we are capable of modifying various glycan processes such as N-glycan elongation and branching, sialic acid capping, and O-glycan core extension, and achieving customized glycoprotein products and other glycoconjugates. For further details of our mammalian cell glycoengineering services, please refer to our N-linked Glycoengineering and O-linked Glycoengineering services in mammalian cells.

Advantages of Our Services

• Optional glycoengineering strategies for tailored glycan patterns
• Cutting-edge techniques for gene regulation and precise genome editing
• Customized glycoengineering and manipulating for specific glycoprotein products
• Professional team with extensive experience in glycoengineering

Staying at the forefront of technological advancements, Creative Biolabs is committed to offering comprehensive glycoengineering services in mammalian cells, providing high-quality glycoprotein products for various applications. If you have any specific inquiries or require further information regarding your glycoengineering needs, please don't hesitate to contact us or send an inquiry directly.

Reference

1. Kontoravdi, Cleo, and Ioscani Jimenez del Val. "Computational tools for predicting and controlling the glycosylation of biopharmaceuticals." Current opinion in chemical engineering 22 (2018): 89-97.

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