Yeast Glycoengineering Services

Yeast Glycoengineering for Glycoprotein Production

Yeast strains have gained widespread industrial utility in the synthesis of recombinant proteins, due to several factors like the ease of scalability, high yields of secreted protein, and culturing in media without animal-derived growth factors. Yeasts possess the capability to perform critical post-translational modifications, including N- and O-glycosylation. Moreover, the pathways for initial glycan transfer are common from yeast to mammals, with highly homologous enzymes participating in these processes. Advancements in glycoengineering have enabled the development of yeast strains capable of replicating critical glycosylation pathways in mammals, including both N-glycosylation and specific forms of O-glycosylation. These glycoengineered yeast strains have the potential to produce humanized glycoproteins at scale.

Fig.1 Overview of N- and O-glycan engineering in yeast.¹

Yeast Glycoengineering Services at Creative Biolabs

With years of experience in Glycoengineering Services, Creative Biolabs has pioneered diverse modulations regarding N- and O-linked glycoengineering in yeast through the application of Genetic Engineering Techniques, aiming to develop "humanized" yeast capable of producing tailored human-like glycans on glycoproteins. We achieve this mainly by stable knocking out or efficiently inhibiting certain native enzymes in yeast to eliminate unwanted yeast-specific glycans, combined with the introduction of different heterologous enzymes responsible for glycan synthesis to reconstruct and humanize glycosylation pathways in yeast.

Fig.2 Workflow of our glycoengineering services in yeast. (Creative Biolabs Original)

Our Specific Services in Yeast Glycoengineering

Our glycoengineering approaches hold great promise for the production of glycoproteins with customized glycan profiles in yeast, we provide both N- and O-linked glycoengineering services, leveraging these approaches to meet the diverse needs of our clients.

Fig.3 Our services in yeast glycoengineering. (Creative Biolabs Original)

Applications

Fig.4 Commercial glycoprotein products produced in yeast. (Creative Biolabs Original)

Creative Biolabs is your trusted partner for comprehensive glycoengineering services in yeast to meet diverse recombinant production needs. With our team of experts and cutting-edge technologies, we are well-equipped to assist you in this field. If you are seeking high-quality and customized glycoengineering services, we highly encourage you to contact us for top-notch support and tailored solutions.

Reference

1. Hamilton, Stephen R., and Dongxing Zha. "Progress in yeast glycosylation engineering." Glyco-Engineering: Methods and protocols (2015): 73-90.

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