Insect Cell Glycoengineering Services

Why Glycoengineering in Insect Cell?

Insect expression systems, combined with baculovirus expression vector systems, have gained widespread popularity for recombinant protein production due to their cost-effectiveness and high yield for secreted protein. Insect cells possess the capacity for posttranslational modifications including both N- and O-glycan processing as mammalian cells. This capability makes insect cells attractive as expression hosts for producing recombinant glycoproteins. However, the endogenous glycan processing pathways in insects are less extensive than in mammalian cells, such as insect cells typically produce simpler and truncated paucimannose N-glycans and lack the ability to perform terminal sialylation. Glycoengineering in insect cells, such as the introduction of humanized glycosylation pathways, enables the production of recombinant glycoproteins featuring human-type or customized glycans.

Fig.1 Glycoengineering in insect-based expression systems.¹

Insect Cell Glycoengineering Services at Creative Biolabs

With years of experience in Glycoengineering Services, Creative Biolabs has been at the forefront of efforts to address the challenges associated with glycosylation in insect expression systems. We offer comprehensive services in insect-based glycoengineering, which primarily involve eliminating insect-specific pathogenic glycans and introducing mammalian glycosylation capabilities into insect cells. Additionally, the insect-baculovirus cell system is a binary system consisting of two different platforms. Creative Biolabs provides glycoengineering services not only in insect cells but also in baculovirus expression vectors, based on various strategies. These services are designed to facilitate the high-yield production of humanized proteins with customized glycosylation profiles.

Strategies of Glycoengineering in Insect-baculovirus Expression System

Glycoengineering in insect cell
Efficient knockout of genes responsible for pathogenic glycan structures.
High-level expression of mammalian glycosyltransferases as well as the target protein.
Glycoengineering in baculovirus expression vector
Expression of mammalian glycosyltransferases.
Expression of the target protein.

Co-infection of baculoviruses encoding mammalian glycosyltransferases and the target protein.
Single-infection of the baculovirus encoding glycosyltransferases and the target protein simultaneously.

Techniques for Insect Cell Glycoengineering

Creative Biolabs has developed powerful tools and methods for glycoengineering in insect-based expression systems. These methods encompass Gene Silencing through RNA interference (RNAi), complete gene suppression via stable gene knockout, and achieving high-level gene Overexpression. Our repertoire of Precise Genome Editing tools has been effectively employed for site-specific modifications. These tools have the capability to facilitate not just gene insertions but also deletions and mutations.

Equipped with these cutting-edge techniques, Creative Biolabs is confident in providing high-quality glycoengineering services, particularly in N-linked Glycoengineering in Insect Cell. It's worth noting that although N-linked glycosylation has been extensively engineered in insect cells, O-linked glycoengineering in insect cells is still an area that requires further exploration and development.

Features of Our Services

Optional glycoengineering strategies for both baculovirus and insect host.
Cutting-edge techniques for gene regulation and precise genome editing
High-level gene expression and high-yield glycoprotein production
Customized glycoengineering for tailored glycan patterns

Published Data

Technology: Insect cell glycoengineering

Journal: ACS Chemical Biology

IF: 4.634

Published: 2015

Results: In this research, the CRISPR/Cas9 system was employed to modify the fdl gene in Drosophila melanogaster S2 cells, yielding a notably elevated occurrence of site-specific nucleotide insertions and deletions. This editing approach significantly reduced the generation of insect-type Man₃GlcNAc₂ and instead promoted the generation of elongated mammalian-type glycans GlcNAc₂Man₃GlcNAc₂ in S2 cells.

Fig.2 Glycoengineering in insect cells using CRISPR/Cas9 system.²

Drawing on our wealth of experience and cutting-edge technologies, Creative Biolabs is delighted to offer a comprehensive selection of glycoengineering services customized for insect cells. We encourage you to contact us for additional information. Our team of expert scientists is standing by to address any specific requests or questions you may have.

References

Kightlinger, Weston, et al. "Synthetic glycobiology: parts, systems, and applications." ACS synthetic biology 9.7 (2020): 1534-1562.
Mabashi-Asazuma, Hideaki, et al. "Modifying an insect cell N-glycan processing pathway using CRISPR-Cas technology." ACS Chemical Biology 10.10 (2015): 2199-2208.

For Research Use Only.

N-Linked Glycoengineering in Insect Cell