Agonist/Antagonist Assay Services

Agonists

Compounds that bind to a receptor and activate it, producing a biological response. Full agonists activate the receptor to its maximum efficacy, while partial agonists activate the receptor but to a lesser extent.

Antagonists

Compounds that bind to a receptor but do not activate it. Instead, they block or dampen the biological response mediated by agonists. Antagonists can be reversible or irreversible.

Agonist Neurite Outgrowth Assay

Our service provides you with a robust platform to evaluate the effects of various compounds on neuronal growth and differentiation. Utilizing established neuronal cell lines or primary neurons, this assay measures neurite outgrowth in response to agonist treatments, enabling the identification of potential neuroregenerative therapeutics. By quantifying parameters such as neurite length, branching, and overall cell morphology, the service delivers comprehensive insights into the efficacy of test compounds. The assay is adaptable to various experimental conditions, allowing for the assessment of different dosages, treatment durations, and environmental factors.

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Agonist/Antagonist Uptake Assay

Our [3H]-2-deoxy-D-glucose (2-DG) rat transporter cell basal agonist and antagonist uptake assay service is designed to evaluate the functional activity of glucose transporters in cellular models. Utilizing radiolabeled [3H]-2-deoxy-D-glucose, this service measures the uptake of glucose in response to various compounds, including potential agonists and antagonists. The assay can assess the effects of test substances on transporter activity, providing insight into their ability to modulate glucose uptake in rat cell lines. This is particularly valuable for understanding metabolic pathways and the pharmacological profiles of new therapeutic agents targeting glucose metabolism.

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Cell-based Antagonist Assays

Our cell-based antagonist assay services are designed to provide comprehensive analysis of compound efficacy and mechanism of action in a physiological context. Utilizing advanced cellular models, we assess the ability of potential antagonists to inhibit receptor signaling or cellular responses triggered by specific ligands. Our services include detailed screening and validation of compounds, with options for high-throughput formats, tailored assay designs, and multiplexing capabilities. We leverage state-of-the-art technologies, such as live-cell imaging and quantitative assays, to deliver robust data on potency, selectivity, and functional profiles.

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