BiliTrack™ Rat Total Alkaline Phosphatase (ALP) ELISA Kit

CAT#: ITS-0326-WB140
Product Type: ELISA Kit
Species: Rat
Target: Total Alkaline Phosphatase (ALP) enzyme
Brand: BiliTrack™
Development Stage: ADME
ADME Research Domain: Excretion
Short Description
Quantitatively detects total Alkaline Phosphatase (ALP) levels in rat serum, plasma, and biological fluids with high precision.
Brand
BiliTrack™
Development Stage
ADME
ADME Research Domain
Excretion
Description
BiliTrack™ Rat Total ALP ELISA Kit provides a reliable in vitro solution for measuring ALP concentrations in rat biological samples. Utilizing a sensitive sandwich ELISA format, it ensures high specificity and reproducibility, facilitating research in liver function, bone metabolism, and preclinical disease modeling.
Features
High-sensitivity detection with a lower limit of <0.06 ng/ml enables accurate quantification in small-volume rat samples.
Strong specificity with minimal cross-reactivity ensures precise measurement of total ALP amidst complex biological matrices.
Colorimetric sandwich ELISA format compatible with standard 96-well microplate readers provides reliable high-throughput results.
Supplied with lyophilized standards and reagents for enhanced stability, extended shelf-life, and consistent assay performance.
Process Relevance
Monitors total ALP activity to support metabolic, liver, and bone-related research with reproducible and sensitive measurements.
Application Stage
Applicable during preclinical studies, biomarker discovery, and in-process validation in rat models.
Applications
Used to quantify ALP in serum, plasma, and biological fluids, supporting enzyme kinetics studies, disease modeling, ADME profiling studies, and biomarker evaluation research.
Target
Total Alkaline Phosphatase (ALP) enzyme
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Assay Measures
Quantitative
Research Areas
Enzymology, metabolic pathways, hepatology, bone metabolism, pharmacokinetics (ADME), cancer biology, immunology, preclinical biomarker research, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric enzyme activity measurement using a sandwich ELISA approach.
Assay Range
6.25 ng/ml - 400 ng/ml
LOD
< 2.59 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Alkaline phosphatase is an essential enzyme involved in phosphate metabolism, cellular differentiation, and tissue mineralization. Abnormal ALP levels indicate liver dysfunction, bone disorders, or systemic disease, making it a critical biomarker in preclinical research.
Pathways
ALP participates in phosphate hydrolysis and mineralization pathways, influencing bone formation, liver function, and downstream metabolic and immune regulation. Altered activity may reflect disease progression or therapeutic responses.
For Research Use Only | Not For Clinical Use
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