BiliTrack™ Human Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA Kit

CAT#: ITS-0326-WB150
Product Type: ELISA Kit
Species: Human
Target: Human tissue-nonspecific alkaline phosphatase (ALPL) enzyme
Brand: BiliTrack™
Development Stage: ADME
ADME Research Domain: Excretion
Short Description
Quantifies human ALPL levels in biological samples using a sensitive sandwich ELISA platform.
Brand
BiliTrack™
Development Stage
ADME
ADME Research Domain
Excretion
Description
BiliTrack™ Human Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA Kit is a high-performance immunoassay for precise quantification of ALPL in human samples. Utilizing a validated sandwich ELISA format, it enables sensitive detection in serum, plasma, and tissue lysates, supporting studies in bone metabolism, liver function, and disease biomarker evaluation.
Features
High-affinity antibody pairs ensure specific and accurate detection of human ALPL with minimal background interference in complex biological samples.
Sensitive colorimetric readout enables quantification across a wide dynamic range, supporting detection of low-abundance targets.
Ready-to-use kit components including pre-coated plates and calibrated standards ensure consistent, reproducible experimental performance.
Compatible with diverse human sample types, providing flexibility for clinical research and translational study applications.
Process Relevance
Measures ALPL levels to monitor enzymatic activity associated with bone formation and liver function.
Application Stage
Clinical research, biomarker discovery, and translational disease studies.
Applications
Quantitative detection of ALPL in human serum, plasma, and tissues; bone turnover studies; ADME profiling studies; liver disease research; enzyme activity analysis; biomarker validation in clinical samples.
Target
Human tissue-nonspecific alkaline phosphatase (ALPL) enzyme
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Clinical diagnostics, bone metabolism, hepatology, enzymology, pharmacokinetics (ADME), metabolic disease research, etc.
Size
96 tests
Data Output Type
Quantitative determination of ALPL protein concentration via colorimetric ELISA measurement.
Assay Range
3.12 mU/ml - 200 mU/ml
LOD
< 1.34 mU/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Alkaline phosphatase (ALPL) is a key enzyme involved in dephosphorylation and plays a central role in bone mineralization and hepatic function. It is widely expressed in osteoblasts and hepatocytes. Abnormal ALPL levels are linked to bone disorders, liver diseases, and metabolic syndromes.
Pathways
ALPL is involved in phosphate metabolism and biomineralization pathways, promoting hydroxyapatite formation in bone tissue. It operates downstream of osteoblast differentiation signaling and reflects hepatobiliary metabolic activity. Its function is essential for maintaining extracellular matrix integrity and systemic phosphate balance.
For Research Use Only | Not For Clinical Use
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