CelluTest™ Cytotoxicity Assay Kit

CAT#: ITS-1125-YL5
Product Type: Assay Kit
Species: All Species
Brand: CelluTest
Development Stage: Research-Grade Cell-Based Assay Development
Short Description
Measures cell viablity using a colorimetric method.
Brand
CelluTest
Development Stage
Research-Grade Cell-Based Assay Development
Description
This CelluTest™ Cytotoxicity Assay Kit provides a rapid bioluminescent method for evaluating cell membrane integrity in diverse culture systems. The assay quantifies the release of adenylate kinase from damaged cells into the supernatant, utilizing a luciferase-based reaction to produce a light signal. This highly sensitive tool is indispensable for researchers studying cell death mechanisms and evaluating the biological impact of compounds in high-throughput screening protocols.
Features
Employs a non-destructive enzymatic detection method to provide accurate assessments of cell viablity and membrane integrity for high-throughput pharmacological and toxicological screening.
Process Relevance
Evaluating compound toxicity to optimize drug candidate selection and assess cellular robustness.
Application Stage
Drug toxicity testing and cell viablity screening.
Applications
Evaluate compound-induced cell death and membrane integrity in high-throughput screens using cultured primary and immortalized cell lines.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Luminescence
Sample Type
Cell culture supernatants, cell lysates.
Research Areas
Toxicology; Drug Discovery; Oncology
Size
100 tests
Data Output Type
Quantitative cytotoxicity percentage
Assay Time
2 min
LOD
LOD: 50 cells
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
The maintenance of cell membrane integrity is a fundamental indicator of cell health and viablity. Monitoring the release of intracellular components into the extracellular medium is a standard method for assessing the impact of experimental compounds or environmental stressors on cell populations.
Pathways
Cytotoxicity typically involves the activation of apoptosis or necrosis pathways, often triggered by upstream oxidative stress or DNA damage signals. The assay monitors the loss of membrane integrity, a terminal step in several cell death cascades.
For Research Use Only | Not For Clinical Use
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