CytoFinal™ High-Sensitivity Mouse Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit

CAT#: ITS-0326-WB89
Product Type: ELISA Kit
Species: Mouse
Target: Mouse cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively detects cytoplasmic AST (GOT1) levels in mouse serum, plasma, tissues, and cell lysates with high sensitivity.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ High-Sensitivity Mouse AST (GOT1) ELISA Kit provides a reliable, in vitro solution for precise measurement of cytoplasmic AST concentrations across multiple mouse biological samples. Ideal for biomarker validation and metabolic research, the kit enables reproducible, sensitive detection in serum, plasma, tissues, and cell lysates.
Features
Utilizes a sandwich ELISA format with pre-coated antibodies for highly specific detection of mouse cytoplasmic AST across diverse sample types.
Exceptional sensitivity (0.19 ng/mL) with a broad detection range (0.31-20 ng/mL) for accurate quantification
Compatible with serum, plasma, tissue homogenates, and cell lysates, providing versatile experimental and preclinical applications.
Lyophilized standards and stable HRP-conjugated reagents ensure reproducible results and long-term storage convenience.
Process Relevance
Monitors cytoplasmic AST to evaluate liver integrity and metabolic activity in preclinical mouse studies efficiently.
Application Stage
Applicable in preclinical drug discovery, disease modeling, and laboratory quality control assessments.
Applications
Quantitative measurement of AST activity in mouse serum, plasma, tissues, and cell lysates for biomarker discovery, hepatology research, ADME profiling studies, and metabolic pathway studies.
Target
Mouse cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, metabolic disease, enzymology, biomarker development, pharmacokinetics (ADME), preclinical pharmacology, translational research, etc.
Size
96 tests
Data Output Type
Quantitative measurement of cytoplasmic AST levels using a colorimetric ELISA readout.
Assay Range
31.2 pg/ml - 2000 pg/ml
LOD
< 13.9 pg/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Aspartate Aminotransferase (AST/GOT1) is a key cytoplasmic enzyme catalyzing reversible transamination between aspartate and α-ketoglutarate. Its activity reflects hepatocellular integrity and metabolic status, supporting disease modeling and preclinical biomarker studies.
Pathways
AST (GOT1) participates in the malate-aspartate shuttle, linking cytoplasmic and mitochondrial redox balance. Upstream, enzyme activity responds to amino acid availability; downstream, it supports TCA cycle flux and energy metabolism.
For Research Use Only | Not For Clinical Use
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