CytoFinal™ Mouse Aspartate Aminotransferase, Mitochondrial / AST (GOT2) ELISA Kit

CAT#: ITS-0326-WB103
Product Type: ELISA Kit
Species: Mouse
Target: Mitochondrial Aspartate Aminotransferase (AST2) antigen
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures mitochondrial AST (GOT2) concentrations in mouse biological samples with high specificity and sensitivity.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Mouse AST (GOT2) ELISA Kit provides a reliable in vitro method for detecting and quantifying mitochondrial Aspartate Aminotransferase in mouse serum, plasma, tissue homogenates, and other biological fluids. Utilizing a sensitive sandwich ELISA format, the kit ensures precise measurement of AST2 levels with minimal cross-reactivity.
Features
High sensitivity detection down to 0.34 ng/mL enables accurate quantification of low-abundance mitochondrial AST in biological samples.
Excellent specificity ensures negligible cross-reactivity with AST2 analogues, providing reliable and reproducible results.
Lyophilized standards and reagents support long-term storage stability while maintaining assay performance for up to 12 months.
Colorimetric sandwich ELISA format allows easy quantification through HRP-TMB reaction, compatible with standard microplate readers.
Process Relevance
Monitors mitochondrial AST activity to support metabolic, toxicology, and disease-related studies in preclinical mouse models.
Application Stage
Drug discovery, preclinical research, and in-process biomarker validation in mouse studies.
Applications
Suitable for ELISA-based quantification in serum, plasma, tissue homogenates, and other fluids, supporting ADME profiling studies.
Target
Mitochondrial Aspartate Aminotransferase (AST2) antigen
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Mitochondrial metabolism, pharmacokinetics (ADME), liver physiology, toxicology, preclinical pharmacology, metabolic disorder studies, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using colorimetric sandwich ELISA.
Assay Range
0.78 ng/ml - 50 ng/ml
LOD
< 0.34 ng/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Mitochondrial AST (GOT2) catalyzes aspartate and α-ketoglutarate conversion to oxaloacetate and glutamate, linking amino acid metabolism with the TCA cycle. Dysregulation of AST2 is associated with hepatic injury, metabolic disorders, and mitochondrial dysfunction, making it a crucial biomarker in preclinical research.
Pathways
AST2 participates in amino acid metabolism, the malate-aspartate shuttle, and TCA cycle regulation. Upstream substrates include aspartate and α-ketoglutarate, while downstream effects influence energy production and nitrogen metabolism, critical for liver and mitochondrial studies.
For Research Use Only | Not For Clinical Use
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