CytoFinal™ Pig L-Lactate Dehydrogenase A Chain (LDHA) ELISA Kit

CAT#: ITS-0326-WB125
Product Type: ELISA Kit
Species: Pig
Target: Porcine lactate dehydrogenase A chain
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively detects pig LDHA protein levels in biological samples using a sensitive ELISA assay.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Pig L-Lactate Dehydrogenase A Chain (LDHA) ELISA Kit is a highly sensitive sandwich ELISA designed for quantitative detection of LDHA in pig biological samples. This assay enables precise measurement of LDHA levels in serum, plasma, tissue homogenates, and cell culture supernatants, supporting metabolic research, disease modeling, and biomarker studies in porcine systems.
Features
Sensitive sandwich ELISA platform provides accurate quantification of pig LDHA across a broad concentration range in diverse biological samples.
Carefully selected antibody pairs ensure high specificity toward porcine LDHA with minimal cross-reactivity and reduced background interference.
Pre-coated microplate and optimized reagents enable straightforward workflow and reproducible performance across multiple experimental runs.
Compatible with multiple porcine sample matrices including serum, plasma, tissue extracts, and cultured cell supernatants.
Process Relevance
Measures LDHA expression to assess metabolic activity and cellular damage in porcine experimental models.
Application Stage
Biomedical research, veterinary disease studies, drug evaluation, and metabolic pathway investigation.
Applications
Quantitative measurement of pig LDHA in serum, plasma, tissue lysates, and cultured cells for metabolic profiling, cytotoxicity assessment, biomarker analysis, ADME profiling studies, and glycolysis-related research.
Target
Porcine lactate dehydrogenase A chain
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Assay Measures
Quantitative
Research Areas
Metabolic biology, glycolysis research, veterinary biomedical research, inflammation studies, toxicology, pharmacokinetics (ADME), disease biomarker discovery, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using absorbance-based ELISA endpoint detection.
Assay Range
1.56 ng/ml - 100 ng/ml
LOD
0.57 ng/ml
Species
Pig
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase A (LDHA) plays a critical role in anaerobic glycolysis by catalyzing the conversion of pyruvate to lactate while regenerating NAD+. This reaction sustains energy production under hypoxic conditions or high metabolic demand. Abnormal LDHA expression is frequently associated with tissue injury, inflammation, and metabolic remodeling.
Pathways
LDHA functions within the glycolysis and lactate fermentation pathways, facilitating NAD+ regeneration required for continuous glycolytic flux. Its expression is often regulated by hypoxia-responsive signaling such as HIF-mediated transcriptional activation. Elevated LDHA activity contributes to metabolic adaptation in rapidly proliferating or stressed cells.
For Research Use Only | Not For Clinical Use
Online Inquiry