CytoFinal™ Rabbit Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit

CAT#: ITS-0326-WB96
Product Type: ELISA Kit
Species: Rabbit
Target: Detects cytoplasmic Aspartate Aminotransferase (AST, GOT1)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures cytoplasmic AST (GOT1) levels in rabbit biological samples with high specificity and sensitivity.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Rabbit Aspartate Aminotransferase (Cytoplasmic / AST, GOT1) ELISA Kit enables precise in vitro quantification of AST in serum, plasma, tissue homogenates, and other biological fluids. Utilizing a sandwich ELISA format, it offers high sensitivity and reproducibility for research in hepatology, immunology, and cancer biology.
Features
High-sensitivity sandwich ELISA detects AST (GOT1) concentrations as low as 0.25 ng/mL, ensuring reliable quantification in limited samples.
Lyophilized standards and reagents provide stable storage, extended shelf life, and minimal activity loss under recommended conditions.
Colorimetric detection with TMB substrate delivers quantitative results proportional to target concentration, enabling accurate comparative analyses.
Optimized for serum, plasma, tissue homogenates, and diverse biological fluids with minimal cross-reactivity in rabbit samples.
Process Relevance
Monitors cytoplasmic AST (GOT1) activity to assess liver function, tissue damage, and enzymatic process dynamics in research studies.
Application Stage
Applicable in drug discovery, preclinical studies, and in vitro experimental validation.
Applications
Suitable for ELISA-based quantification in serum, plasma, tissue homogenates, and other biological fluids, supporting hepatology, cancer, ADME profiling studies, and immunology research.
Target
Detects cytoplasmic Aspartate Aminotransferase (AST, GOT1)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, tumor immunology, infection immunity, cancer biology, pharmacokinetics (ADME), enzyme and kinase research, etc.
Size
96 tests
Data Output Type
Quantitative measurement of AST (GOT1) levels via colorimetric endpoint detection.
Assay Range
0.625 ng/ml - 40 ng/ml
LOD
< 0.25 ng/ml
Species
Rabbit
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Aspartate Aminotransferase (AST, GOT1) catalyzes reversible transamination between aspartate and α-ketoglutarate, playing a central role in amino acid and energy metabolism. Its cytoplasmic activity is a key biomarker for liver injury, metabolic regulation, and systemic disease states.
Pathways
AST (GOT1) participates in amino acid metabolism, connecting the malate-aspartate shuttle to the TCA cycle. Its activity reflects upstream nitrogen metabolism and downstream energy production efficiency, relevant in hepatic and systemic physiology.
For Research Use Only | Not For Clinical Use
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