P450-Metric™ Cytochrome P450 27B1 (CYP27B1) ELISA Kit

CAT#: ITS-0326-YL13
Product Type: Assay Kit
Species: Mouse, Human
Brand: P450-Metric™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies CYP27B1 using a double-antibody sandwich ELISA.
Brand
P450-Metric™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
This high-performance double-antibody sandwich ELISA kit is developed for the exact quantitative detection of Cytochrome P450 27B1 levels in diverse research matrices. Highlighting outstanding analytical sensitivity, this research-use-only immunoassay allows scientists to meticulously profile extrahepatic endocrine drug metabolism. It acts as a critical analytical resource for evaluating targeted xenobiotic impacts on vitamin D pathways and assessing related pharmacokinetic properties during preclinical ADME screening.
Features
This highly sensitive sandwich ELISA provides accurate quantification of CYP27B1, delivering critical insights into extrahepatic drug metabolism and targeted xenobiotic impact on vitamin D pathways.
Process Relevance
Monitoring CYP27B1 to optimize targeted bioactivation and local tissue distribution of vitamin D-based therapeutics.
Application Stage
Preclinical drug discovery, ADME profiling, and pharmacokinetic research
Applications
This highly specific sandwich ELISA quantifies target protein concentrations across tissue homogenates, cell lysates, and biological fluids.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Sample Type
Serum, plasma, tissue homogenates, cell lysates
Research Areas
ADME; Endocrine Pharmacology; Drug Metabolism; Pharmacokinetics
Size
48 / 96 tests
Data Output Type
Absorbance (OD)
LOD
Mouse: 0.242 ng/mL
Human: 0.054 ng/mL
Species
Mouse, Human
Storage
Upon receipt, please follow the storage recommendations in the USER MANUAL to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Operating as the principal enzyme for synthesizing active vitamin D, Cytochrome P450 27B1 is fundamental to systemic endocrine metabolism. ADME investigations target this enzyme to assess the pharmacokinetic behavior of vitamin D analogs and evaluate off-target xenobiotic effects on calcium homeostasis.
For Research Use Only | Not For Clinical Use
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