CytoFinal™ Rat Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit

CAT#: ITS-0326-WB75
Product Type: ELISA Kit
Species: Rat
Target: Rat alanine aminotransferase 1 (ALT1/GPT)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Sensitive ELISA kit for quantifying ALT1 (GPT) protein levels in rat biological samples.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Rat Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit offers a precise immunoassay for detecting ALT1 in rat serum, plasma, tissue lysates, and cultured cells. Utilizing a high-affinity sandwich ELISA format, the kit delivers reproducible quantification of ALT protein, supporting preclinical liver injury evaluation, metabolic studies, and toxicology research.
Features
Highly specific rat ALT1 antibodies ensure accurate detection with minimal cross-reactivity to related aminotransferase isoforms.
Sensitive sandwich ELISA design allows detection of low-level ALT in complex biological samples.
96-well microplate format enables high-throughput processing compatible with standard ELISA instrumentation.
Pre-calibrated standards and optimized reagents provide consistent, quantitative results across multiple experiments.
Process Relevance
Enables precise measurement of ALT to monitor liver injury, hepatocellular stress, and metabolic enzyme changes in preclinical rat models.
Application Stage
Preclinical research, toxicology assessment, translational metabolism studies, and biomarker evaluation.
Applications
Quantifies ALT protein in rat serum, plasma, tissues, and cultured cells for liver function assessment, metabolic pathway analysis, ADME profiling studies, and toxicology biomarker studies.
Target
Rat alanine aminotransferase 1 (ALT1/GPT)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, toxicology, metabolic disease research, pharmacology, pharmacokinetics (ADME), preclinical biomedical studies, translational metabolism research, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration obtained via absorbance in a sandwich ELISA assay.
Assay Range
0.78 ng/ml - 50 ng/ml
LOD
< 0.27 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
ALT1 catalyzes the transfer of an amino group from alanine to α-ketoglutarate, forming pyruvate and glutamate. In rats, ALT serves as a key biomarker for hepatocellular injury and metabolic function in preclinical studies.
Pathways
ALT1 is involved in the alanine cycle, linking peripheral amino acid metabolism to hepatic gluconeogenesis. Generated pyruvate integrates into the TCA cycle or gluconeogenic pathways, connecting nitrogen metabolism with cellular energy production.
For Research Use Only | Not For Clinical Use
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