CytoFinal™ Rat Aspartate Aminotransferase, Mitochondrial / AST (GOT2) ELISA Kit

CAT#: ITS-0326-WB99
Product Type: ELISA Kit
Species: Rat
Target: Rat mitochondrial Aspartate Aminotransferase (GOT2)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Accurately quantifies mitochondrial AST (GOT2) levels in rat tissues, cell lysates, and biological fluids.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Rat Aspartate Aminotransferase, Mitochondrial / AST (GOT2) ELISA Kit provides a sensitive, reproducible method for measuring GOT2 concentrations in rat samples. Ideal for tissue homogenates, serum, and cell lysates, this kit supports metabolic and mitochondrial research by enabling reliable quantification of enzymatic activity in preclinical rat models.
Features
Sandwich ELISA format delivers high specificity and reproducibility for rat mitochondrial GOT2 detection across various sample types.
Sensitive colorimetric readout enables detection within a broad concentration range, ensuring precise quantitative analysis.
Lyophilized standards and reagents provide long-term stability, reducing variability and ensuring consistent assay performance.
Complete 96-well kit supports high-throughput experiments with streamlined workflow and reproducible results across multiple studies.
Process Relevance
Enables monitoring of mitochondrial AST activity to support preclinical metabolic and biomarker studies in rats.
Application Stage
Preclinical research, metabolic studies, and in-process biomarker validation.
Applications
Quantitative assessment of rat mitochondrial GOT2 in ELISA assays using tissue homogenates, serum, or cell lysates for downstream ADME profiling studies.
Target
Rat mitochondrial Aspartate Aminotransferase (GOT2)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Mitochondrial metabolism, liver disease, cardiovascular research, neurodegeneration, biomarker discovery, pharmacokinetics(ADME), metabolic syndrome, etc.
Size
96 tests
Data Output Type
Quantitative enzyme concentration measurement using colorimetric detection.
Assay Range
0.78 ng/ml - 50 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
GOT2 is a mitochondrial aminotransferase critical for the malate-aspartate shuttle and amino acid metabolism. Its activity informs energy metabolism, liver function, and mitochondrial health, serving as a biomarker in preclinical rat studies.
Pathways
GOT2 participates in the malate-aspartate shuttle, mediating amino group transfer between glutamate and oxaloacetate. It regulates TCA cycle flux, NADH/NAD+ balance, and downstream energy metabolism, impacting cellular metabolic homeostasis.
For Research Use Only | Not For Clinical Use
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