CytoFinal™ Rat Lactate Dehydrogenase A (LDHA) ELISA Kit

CAT#: ITS-0326-WB120
Product Type: ELISA Kit
Species: Rat
Target: Rat Lactate Dehydrogenase A (LDHA) protein
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies Rat LDHA concentrations in tissues, cell lysates, and biological fluids with high specificity.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Rat LDHA ELISA Kit enables precise in vitro measurement of Lactate Dehydrogenase A levels in rat tissue homogenates, cell lysates, and other biological fluids. Utilizing a sensitive sandwich ELISA format, it provides robust and reproducible quantification across a broad dynamic range.
Features
High specificity for Rat LDHA ensures minimal cross-reactivity and accurate detection in complex biological samples.
Colorimetric detection with TMB substrate provides clear visual readout, compatible with standard 96-well microplate readers.
Broad assay range from 0.31-20 ng/mL with sensitivity of 0.19 ng/mL, supporting low-abundance protein quantification.
Lyophilized standards and reagents enhance stability, enabling consistent performance and long-term storage at 4℃
Process Relevance
Supports monitoring of LDHA activity to optimize metabolic studies and disease-related research in rat models.
Application Stage
Drug discovery, preclinical metabolic research, and in-process biological sample analysis.
Applications
Quantitative measurement of LDHA in rat tissue homogenates, cell lysates, plasma, serum, and other biological fluids for ADME profiling studies.
Target
Rat Lactate Dehydrogenase A (LDHA) protein
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Metabolic pathway analysis, oncology research, cardiovascular physiology, diabetes, pharmacokinetics (ADME), other metabolic disorder studies, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using colorimetric sandwich ELISA.
Assay Range
0.31 ng/ml - 20 ng/ml
LOD
0.19 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
LDHA catalyzes the conversion of pyruvate to lactate in anaerobic glycolysis, playing a central role in energy metabolism. Its dysregulation is implicated in cancer, ischemia, and metabolic disorders, making accurate quantification essential for mechanistic and translational studies.
Pathways
LDHA functions within glycolytic and lactate metabolism pathways. It operates downstream of pyruvate formation, influencing NAD+/NADH balance and feeding into the Cori cycle and cellular energy homeostasis.
For Research Use Only | Not For Clinical Use
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