GuardianOx™ Phosphate Assay Kit

CAT#: ITS-1125-HM89
Product Type: Assay Kit
Species: All Species
Brand: GuardianOx
Development Stage: Research-Grade Oxidative Stress Assay Development
Short Description
Malachite green method for inorganic phosphate quantification.
Brand
GuardianOx
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
The GuardianOx™ Phosphate Assay Kit uses a malachite green formulation to provide high sensitivity for measuring ATPase and phosphatase activity in kinase screens. This assay is essential for validating drug targets involved in signal transduction and energy metabolism. It offers a universal readout for phosphate-releasing enzymes, supporting high-throughput inhibitor screening in drug discovery.
Features
Malachite green formulation; High sensitivity for measuring ATPase/Phosphatase activity in kinase screens.
Process Relevance
Kinase/ATPase Assay Readout
Application Stage
Hit-to-Lead Screening
Applications
Bone mineral metabolism and renal phosphate handling.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 620 nm (malachite green)
Sample Type
Serum, urine, saliva, sweat, tissue culture, food, environment, samples
Research Areas
Nephrology; Bone Biology
Size
500 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 30 min
LOD
3 µg/dL (0.3 µM)
Species
All Species
Storage
Store at 4 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid freezing unless otherwise specified. During use, minimize prolonged exposure to room temperature and return components promptly to 2-8 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Inorganic phosphate is a structural component of bone and ATP. Serum phosphate levels are regulated by the kidneys; hyperphosphatemia is a common complication of chronic kidney disease.
For Research Use Only | Not For Clinical Use
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