Short Description
Malachite green method for inorganic phosphate quantification.
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
The GuardianOx™ Phosphate Assay Kit uses a malachite green formulation to provide high sensitivity for measuring ATPase and phosphatase activity in kinase screens. This assay is essential for validating drug targets involved in signal transduction and energy metabolism. It offers a universal readout for phosphate-releasing enzymes, supporting high-throughput inhibitor screening in drug discovery.
Features
Malachite green formulation; High sensitivity for measuring ATPase/Phosphatase activity in kinase screens.
Process Relevance
Kinase/ATPase Assay Readout
Application Stage
Hit-to-Lead Screening
Applications
Bone mineral metabolism and renal phosphate handling.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 620 nm (malachite green)
Sample Type
Serum, urine, saliva, sweat, tissue culture, food, environment, samples
Research Areas
Nephrology; Bone Biology
Datasheet