This model focuses on rapid T-cell engraftment, providing a high-throughput platform to evaluate T-cell engaging bispecifics and checkpoint inhibitors with superior speed and efficiency in research.
Learn More →Humanized Model Services
Creative Biolabs provides specialized Humanized Models for advanced preclinical research, offering functional human immune or metabolic systems in vivo. Clients gain access to highly predictive translational data that clarifies drug efficacy and mechanism of action (MoA) in a human-relevant context. Our tailored service ensures efficient target validation and candidate screening, accelerating your research milestones with robust, decision-ready scientific results.
Introduction What We Can Offer How We Can Help Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us
Background
Humanized Models represent the cutting edge of translational science, offering a crucial step in de-risking novel therapeutics. By incorporating functional human immune or metabolic systems into immunodeficient mice, these platforms overcome the species barrier inherent to traditional preclinical testing. This enables accurate assessment of human-specific drug responses, supporting the conclusion that utilizing such predictive models is essential for modern drug development and scientific confidence.
Discover how we can help - Request a consultation.
Fig.1 Challenging humanized mice with EBV and KSHV infection.1
What We Can Offer
The major drawback of cell line-derived xenografts (CDX) and patient-derived xenografts (PDX) models is the lack of an immunocompetent host, while immunotherapy is based on manipulation of the immune system to fight cancer. Here, as a breakthrough platform, humanized mouse models can generate functional human immune systems with expanding ability. Our scientists established the following humanized mouse models to support your immuno-oncology research.
CD34+ Humanized Mice
To facilitate the analysis of the safety and efficacy outcomes of potential novel agents modulating the immune system, Creative Biolabs offers humanized CD34+ mice as a robust in vivo model for long-term immuno-oncology studies.
It has been characterized as a functional human immune system when this in vivo model is implanted with cord blood-derived hematopoietic stem cells (HSC), and robust T-cell maturation and T-cell-dependent inflammatory responses have also been confirmed.
PBMC Humanized Mice
Humanized PBMC mice are another in vivo model with the highest engraftment rate using adult peripheral blood mononuclear cells. It has been characterized as a functional human immune system displaying strong effector and memory T cells and NK cells in short-term studies. Creative Biolabs offers comprehensive, innovative PBMC-related mouse models for immune-oncology studies, such as the following model types:
Human PBMC-T Mouse Model
Human PBMC-B Mouse Model
Engineered for the study of B-cell malignancies, this model enables precise evaluation of B-cell depletion and targeting therapies within an active human immune environment.
Learn More →Human PBMC-NK Mouse Model
Specialized for Natural Killer cell research, this platform allows for the assessment of NK-mediated cytotoxicity and ADCC effects, essential for validating novel antibody-based discovery programs.
Learn More →Human PBMC-MHC KO Mouse Model
By utilizing MHC-deficient mice, this model significantly delays GvHD, extending the study window for evaluating long-term therapeutic efficacy while maintaining a functional human immune system.
Learn More →Humanized mice harbor human immune cells, which can be induced to produce an immune response by immunotherapy. This kind of animal model has been used to detect compounds that manipulate the immune system to attack cancer. It makes up for the shortcomings of patient-derived and traditional xenografts, which lack immunocompetent hosts. These humanized mouse models can be combined with the following tools to enhance the study outcomes and advance your programs.
| • PDX models | • Flow cytometry | • Optical imaging |
| • Histopathology | • Biomarker screening | |
How Creative Biolabs' service Can Assist Your Projects
Why Choose Us
| Key Advantages | Unique Features |
|---|---|
| Unrivaled Engraftment Quality | Specialized expertise in handling and transplanting human cells, resulting in consistently high levels of functional human immune cell reconstitution, especially crucial for BRGSF and NOG recipient strains. |
| Multi-Modal Readout Integration | Beyond standard tumor volume, we integrate advanced analytical tools like LBA (cytokine/biomarker expression) and optical imaging with flow cytometry, delivering deep MoA insights. |
| Commitment to Human-Relevance | The platform is proven; published data show highly successful CAR T cell products were first validated in similar humanized systems, demonstrating our commitment to models with high human-relevance predictive power. |
Discover how a partnership with us can streamline your research - Get a quote today.
Customer Reviews
-
Predictive PK/PD
The implementation of Creative Biolabs' humanized models substantially enhanced the prediction of human-specific drug metabolism (DDI) within our small molecule oncology program. Specifically, the genetic humanization platform provided accurate identification of a potential hepatotoxicity risk, an outcome undetected by conventional murine models. - J**n M***r. -
MoA Clarity in IO
Using Creative Biolabs' humanized models in our research has significantly improved the quantification of tumor-infiltrating lymphocyte (TIL) fractions via FACS following our checkpoint inhibitor treatment. This specific, human-relevant readout provided the MoA clarity required for our next-stage research, far surpassing traditional xenograft data. - S***h L***s.
FAQs
How do you ensure the human immune cells remain functional in the mouse host?
We use highly immunodeficient recipient strains (like NOG, NSG, and BRGSF), which lack host immune function, preventing rejection. Furthermore, our protocols include comprehensive quality control measures, such as flow cytometry confirmation of key human cell populations (e.g., CD45+, CD3+, CD19+) and cytokine profile analysis, ensuring functionality throughout the study.
Can I test my compound's effect on human drug metabolism using these models?
Yes. While standard cellular humanization focuses on immunity, our genetic humanization models incorporate humanized liver enzymes (e.g., Cyp450 enzymes), which are specifically designed to predict human drug metabolism (ADME) and prevent drug-drug interactions (DDI). This is a specialized service we highly recommend for small molecule therapies.
Related Services
Patient Derived Xenografted Models
We facilitate high-fidelity tumor model acquisition and initial target validation, ensuring biological relevance prior to complex human immune system engraftment in mice.
Learn More →In Vivo DMPK Service
We provide dedicated in vitro and in vivo profiling to complement humanized models, accurately predicting drug metabolism and absorption during early-stage discovery.
Learn More →How to Contact Us
Creative Biolabs has validated all the models available, and we are committed to accelerating your immuno-oncology studies and offering the most appropriate in vivo model for next-stage preclinical testing. Please contact us for more information.
Reference
- Münz, Christian. "Probing Reconstituted Human Immune Systems in Mice With Oncogenic γ-Herpesvirus Infections." Frontiers in immunology vol. 11 581419. 9 Sep. 2020. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fimmu.2020.581419
Download our brochureLoading case studies...
Online Inquiry